Updated on 2022-10-28: We won Best Part Collection this year.
Our parts could be divided into three major groups:
(I) parts were designed for ribozyme-assisted polycistronic co-expression, mainly for crt genes
- BBa_K4162005
- BBa_K4162006
- BBa_K4162009 - BBa_K4162021
- BBa_K4162101 - BBa_K4162117, BBa_K4162118, BBa_K4162119
(II) parts are designed for the last two steps of our retinol production, and TEARS
- BBa_K4162003
- BBa_K4162004
- BBa_K4162007
- BBa_K4162008
- BBa_K4162022 - BBa_K4162027
- BBa_K4162031
- BBa_K4162032
(III) parts are short peptide linkers, tags and fluorescent proteins required in our project
- BBa_K4162001
- BBa_K4162002
- BBa_K4162033
- BBa_K4162034
The above three groups together constitute a coherent part collection for the production of β-carotene, retinaldehyde and retinol. Below list all the parts we built for our project. Please click the name to see deails on the Registry.
| Part Name | Description | Part Type | Designer(s) | Length | |
|---|---|---|---|---|---|
| BBa_K4162001 | StayGold = (n2)oxStayGold(c4)v2.0 | Coding | Weiwen Chen | 711 bp | |
| BBa_K4162002 | linker between BCMO and StayGold, hydrophobic | Coding | Weiwen Chen | 54 bp | |
| BBa_K4162003 | ybbO coding for retinol dehydrogenase, codon optimized for E. coli | Coding | Yujian Song | 810 bp | |
| BBa_K4162004 | Gene coding for BCMO from uncultured marine bacteria | Coding | Weiwen Chen | 828 bp | |
| U | BBa_K4162005 | Hammerhead ribozyme | RNA | Weiwen Chen | 57 bp |
| BBa_K4162006 | T7_RBS | RBS | Weiwen Chen | 17 bp | |
| BBa_K4162007 | MCP | Coding | Weiwen Chen | 336 bp | |
| BBa_K4162008 | tdMCP | Coding | Weiwen Chen | 690 bp | |
| BBa_K4162009 | ribozyme + B0_RBS + crtE | DNA | Weiwen Chen | 991 bp | |
| BBa_K4162010 | ribozyme + T7_RBS + crtE | DNA | Weiwen Chen | 993 bp | |
| BBa_K4162011 | ribozyme + J6_RBS + crtE | DNA | Weiwen Chen | 981 bp | |
| BBa_K4162012 | ribozyme + B0_RBS + crtB | DNA | Weiwen Chen | 1012 bp | |
| BBa_K4162013 | ribozyme + T7_RBS + crtB | DNA | Weiwen Chen | 1014 bp | |
| BBa_K4162014 | ribozyme + J6_RBS + crtB | DNA | Weiwen Chen | 1002 bp | |
| BBa_K4162015 | ribozyme + B0_RBS + crtI | DNA | Weiwen Chen | 1561 bp | |
| BBa_K4162016 | ribozyme + T7_RBS + crtI | DNA | Weiwen Chen | 1563 bp | |
| BBa_K4162017 | ribozyme + J6_RBS + crtI | DNA | Weiwen Chen | 1551 bp | |
| BBa_K4162018 | ribozyme + B0_RBS + crtY | DNA | Weiwen Chen | 1231 bp | |
| BBa_K4162019 | ribozyme + T7_RBS + crtY | DNA | Weiwen Chen | 1233 bp | |
| BBa_K4162020 | ribozyme + J6_RBS + crtY | DNA | Weiwen Chen | 1221 bp | |
| BBa_K4162021 | ribozyme+RBS+CDS module: crtIYEB | DNA | Weiwen Chen | 4896 bp | |
| BBa_K4162022 | ribozyme + T7_RBS + BCMO | DNA | Weiwen Chen | 909 bp | |
| BBa_K4162023 | ribozyme + T7_RBS + ybbO | DNA | Weiwen Chen | 891 bp | |
| BBa_K4162024 | ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO | DNA | Weiwen Chen | 1800 bp | |
| BBa_K4162025 | ribozyme + T7_RBS + ybbO + ribozyme + T7_RBS + BCMO | DNA | Weiwen Chen | 1800 bp | |
| BBa_K4162026 | ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP | DNA | Weiwen Chen | 2508 bp | |
| BBa_K4162027 | ribozyme + T7_RBS + ybbO-tdMCP | DNA | Weiwen Chen | 1599 bp | |
| BBa_K4162028 | ribozyme + T7_RBS + ybbO-tdMCP-EGFP | DNA | Weiwen Chen | 2340 bp | |
| BBa_K4162029 | ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP | DNA | Weiwen Chen | 3249 bp | |
| BBa_K4162031 | ribozyme + T7_RBS + BCMO-StayGold | DNA | Weiwen Chen | 1674 bp | |
| BBa_K4162032 | ribozyme + T7_RBS + BCMO-StayGold + ribozyme + T7_RBS + ybbO | DNA | Weiwen Chen | 2565 bp | |
| BBa_K4162033 | linker between gene ybbO and tdMCP | DNA | Weiwen Chen | 18 bp | |
| BBa_K4162034 | linker between tdMCP and EGFP | DNA | Weiwen Chen | 18 bp | |
| BBa_K4162101 | ribozyme+RBS+CDS module: crtIY | Coding | Weiwen Chen | 2796 bp | |
| BBa_K4162102 | ribozyme+RBS+CDS module: crtEI | Coding | Weiwen Chen | 2556 bp | |
| BBa_K4162103 | ribozyme+RBS+CDS module: crtBE | Coding | Weiwen Chen | 2007 bp | |
| BBa_K4162104 | ribozyme+RBS+CDS module: crtEB | Coding | Weiwen Chen | 2007 bp | |
| BBa_K4162105 | ribozyme+RBS+CDS module: crtYB | Coding | Weiwen Chen | 2247 bp | |
| BBa_K4162106 | ribozyme+RBS+CDS module: crtIB | Coding | Weiwen Chen | 2577 bp | |
| BBa_K4162107 | ribozyme+RBS+CDS module: crtEY | Coding | Weiwen Chen | 2226 bp | |
| BBa_K4162108 | ribozyme+RBS+CDS module: crtEBI | Coding | Weiwen Chen | 3570 bp | |
| BBa_K4162109 | ribozyme+RBS+CDS module: crtIEB | Coding | Weiwen Chen | 3570 bp | |
| BBa_K4162110 | ribozyme+RBS+CDS module: crtBEY | Coding | Weiwen Chen | 3240 bp | |
| BBa_K4162111 | ribozyme+RBS+CDS module: crtBEI | Coding | Weiwen Chen | 3570 bp | |
| BBa_K4162112 | ribozyme+RBS+CDS module: crtIYB | Coding | Weiwen Chen | 3810 bp | |
| BBa_K4162113 | ribozyme+RBS+CDS module: crtEBY | Coding | Weiwen Chen | 3240 bp | |
| BBa_K4162114 | ribozyme+RBS+CDS module: crtYEB | Coding | Weiwen Chen | 3240 bp | |
| BBa_K4162115 | ribozyme+RBS+CDS module: crtYBE | Coding | Weiwen Chen | 3240 bp | |
| BBa_K4162116 | ribozyme+RBS+CDS module: crtEIY | Coding | Weiwen Chen | 3789 bp | |
| U | BBa_K4162117 | ribozyme+RBS+CDS module: crtYEBI | DNA | Weiwen Chen | 4836 bp |
| U | BBa_K4162118 | ribozyme+RBS+CDS module: crtEBIY | DNA | Weiwen Chen | 4848 bp |
| BBa_K4162119 | ribozyme+RBS+CDS module: crtYBEI | DNA | Weiwen Chen | 4836 bp |