Basic Parts
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375003 | Regulatory | J23101* | Strong constitutive promoter, derived from J23101. |
| BBa_K4375004 | Signalling | BLADE transcription factor | BLADE, AraC transcription factor engineered to be light-activable. |
| BBa_K4375005 | Coding | anti-EGFR Nanobody (7D12) | EGFR-specific nanobody. |
| BBa_K4375006 | Coding | anti-CEA Nanobody (C43) | CEA-specific (carcinoembryonic antigen) Nanobody is optimized for production in E. coli. |
| BBa_K4375008 | Tag | miRFP670 nano | Small (17 kDa), near-infrared emitting fluorescent protein. |
| BBa_K4375009 | Linker | GS linker with BamHI restriction site | A 13 aa long GS linker with a BamHI restriction site at its C-terminal. |
| BBa_K4375010 | Linker | GS linker | A 13 aa long flexible GS linker. |
| BBa_K4375011 | Linker | Llama immunoglobulin IgA hinge linker | Ideal linker for Nanobody fusion derived from llama. |
| BBa_K4375012 | Coding | Intimin domain for surface display (with anti-E epitope tag) | This protein contains the N-terminal domain of intimin, which is a bacterial adhesion factor, D0 an Ig-like protein, and an E epitope tag. Intimin beta-barrel transmembrane domain anchors the construct into the outer membrane of the bacteria. |
| BBa_K4375013 | Tag | Myc epitope tag with SpHI restriction site | |
| BBa_K4375014 | Tag | Myc epitope tag |
Composite Parts
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375016 | Device | anti-EGFR Nanobody Display System | This construct is meant for detecting cancer cells via fluorescence that expresses EGFR receptors by bacterial cells. |
| BBa_K4375017 | Device | anti-CEA Nanobody Display System | This construct is meant for detecting cancer cells via fluorescence that expresses CEA by bacterial cells. |
| BBa_K4375019 | Device | Blue light-inducible production of ClyA (BLADE) | |
| BBa_K4375021 | Device | Blue light-inducible production of mCherry (BLADE) |
Improved Parts
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375007 | Coding | Codon optimized INPNC for surface display | INPNC (codes for N- and C- terminal domain of Ice Nucleation Protein (INP)) optimized for production in E. coli. |
| BBa_K4375018 | Device | MiniNano Display System | The MiniNano is a compact construct meant for EGFR detection via fluorescence. |
Primers
| Registry | Type | Name |
|---|---|---|
| BBa_K4375022 | Primer | Forward primer for sfGFP |
| BBa_K4375023 | Primer | Reverse primer for sfGFP |
| BBa_K4375024 | Primer | Forward primer for BLADE |
| BBa_K4375025 | Primer | Reverse primer for J23101-BLADE-ClyA |
| BBa_K4375026 | Primer | Reverse primer for J23101-BLADE-mCherry |
| BBa_K4375027 | Primer | Forward primer for Intimin |
| BBa_K4375028 | Primer | Reverse primer for Intimin |