Light-inducible protein production
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375004 | Signalling | BLADE transcription factor | BLADE, AraC transcription factor engineered to be light-activable. |
| BBa_K4375019 | Device | Blue light-inducible production of ClyA (BLADE) | |
| BBa_K4375021 | Device | Blue light-inducible production of mCherry (BLADE) |
Tumour detecting display system
Nanobodies
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375005 | Coding | anti-EGFR Nanobody (7D12) | EGFR-specific nanobody. |
| BBa_K4375006 | Coding | anti-CEA Nanobody (C43) | CEA-specific (carcinoembryonic antigen) Nanobody is optimized for production in E. coli. |
Display Systems
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375012 | Coding | Intimin domain for surface display (with anti-E epitope tag) | This protein contains the N-terminal domain of intimin, which is a bacterial adhesion factor, D0 an Ig-like protein, and an E epitope tag. Intimin beta-barrel transmembrane domain anchors the construct into the outer membrane of the bacteria. |
| BBa_K4375007 | Coding | Codon optimized INPNC for surface display | INPNC (codes for N- and C- terminal domain of Ice Nucleation Protein (INP)) optimized for production in E. coli. |
Tumour detection apparatus
| Registry | Type | Name | Description |
|---|---|---|---|
| BBa_K4375016 | Device | anti-EGFR Nanobody Display System | This construct is meant for detecting cancer cells via fluorescence that expresses EGFR receptors by bacterial cells. |
| BBa_K4375017 | Device | anti-CEA Nanobody Display System | This construct is meant for detecting cancer cells via fluorescence that expresses CEA by bacterial cells. |
| BBa_K4375018 | Device | MiniNano Display System | The MiniNano is a compact construct meant for EGFR detection via fluorescence. |