The part consists of the 1000 bp upstream of Formate Dehydrogenase 1 (FDH1) CDS from S. cerevisiae. FDH1 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in A. niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.

The part consists of the 1000 bp upstream of Formate Dehydrogenase 2 (FDH2) CDS from S. cerevisiae. FDH2 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in A. niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.

The part consists of the 1000 bp upstream of Formate Dehydrogenase (FDH) CDS from A. niger. Since FDH genes have been shown to be upregulated in response to furfural in S cerevisiae, the part was utilised to test if anFDH promoter is also responsive to furfural. Quantification by qPCR of the FDH transcript and mCherry transcript from a transiently expressed BBa_K4129005 (BBa_K4129002 regulating mCherry reporter), showed that BBa_K4129002 is induced by furfural both in its native regulatory enviroment and when used in syntetic expression cassettes.

This coding sequence produces a putative NAD-dependent aldehyde dehydrogenase named arz7774, identified in Amorphotheca resinae. It has been shown to be upregulated in Amorphotheca resinae while induced by furfuryl alcohol, a derivative of furfural and it is hypothesised to convert furfural to furoic acid, which is a part of a larger conversion pathway to channel furfural towards the citric acid cycle.

This coding sequence produces a NADH-dependent oxidoreductase from E coli, and has been shown to reduce furfural to furfuryl alcohol.

This coding sequence produces a putative FAD-dependent oxidoreductase from Cupriavidus basilensis. This enzyme is hypothesised to oxidise both furfural and furfuryl alcohol to furoic acid, which is a part of a larger conversion pathway to channel furfural towards the citric acid cycle.

Protospacer to guide cas9 to insertion site #1 on the genome of A. niger

Protospacer to guide cas9 to insertion site #1 on the genome of A. niger

Protospacer to guide cas9 to insertion site #3 on the genome of A. niger

Strong constitutive A. nidulans gpdA promoter (PgpdA), from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503723/

This is a plasmid for transient expression in A. niger containing an E. coli ori, an ampicillin selection marker, a pyrG auxotrophyc selection marker and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.

FunsTF02 is a synthetic transcription factor (sTF) based on a sensor of benzoic acid derivatives (sBAD), which is a sTF in S. cerevisiae (Castaño-Cerezo et. al (2020)). FunsTF02 deviates from sBAD, in that it has a nuclear localization signal (NLS) and is codon optimised to A. niger. FunsTF02 is a fusion protein consisting of the DNA-binding domain from LexA, the ligand sensing domain from HbaR, transactivation domain B112 and the nuclear localization signal (NLS) SV40.

The designed function of FunsTF02 is to be used as a transcription factor that can initiate transcription from the 6xLexO minimal promoter (BBa_K4129115). This sTF is designed to be the sensing part of a biosensor.

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)).

The transactivation domain B112 is from E. coli, and it was experimentally proven to initiate transcription of a synthetic promoter in S. cerevisiae (Ottoz et. al (2014)). The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport og the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF04 is a synthetic transcription factor (sTF). FunsTF04 is designed to function as a transcription factor that can initiate transcription from the 6xLexO minimal promoter (BBa_K4129115). This sTF is designed to be the sensing part of a biosensor.

FunsTF04 is a fusion protein consisting of the DNA-binding domain from LexA, the ligand sensing domain from Hmox1, transactivation domain B112 and the nuclear localization signal (NLS) SV40. The linker between LexA and Hmox1 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF05 was codon optimised to A. niger.

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)), and it is the DNA binding domain, which is interacting with LexO, that is used in FunsTF04. Hmox1 is the human heme oxygenase 1, which is the enzyme that initiates cleavage of heme (Tenhunen et al. (1969)). This enzyme was, despite the seemingly unrelated context, computationally shown to bind furfural (Santhakumar et al (2021)).

The transactivation domain B112 is from E. coli, and was experimentally proven to initiate transcription of a synthetic promoter in S. cerevisiae (Ottoz et. al (2014)). The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport og the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF05 is a synthetic transcription factor (sTF). FunsTF05 is designed to function as a transcription factor. In theory, FunsTF05 should be able to interact with an inducer and this interaction will facilitate transcription from the 6xLexO minimal promoter (BBa_K4129115). This sTF can be the sensing part of a biosensor.

FunsTF05 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain Hmox1, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and Hmox1 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF05 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)), and it is the DNA binding domain interacting with LexO that is used in FunsTF05. Hmox1 is the human heme oxygenase 1, which is the enzyme that initiates cleavage of heme (Tenhunen et al. (1969)). This enzyme was, despite the seemingly unrelated context, computationally shown to bind furfural (Santhakumar et al (2021)).

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF16 is a synthetic transcription factor (sTF) based on sensor of benzoic acid derivatives (sBAD), which is a sTF in S. cerevisiae (Castaño-Cerezo et. al (2020)). FunsTF16 deviates from sBAD, because it has an nuclear localization signal (NLS) and is codon optimised to A. niger. FunsTF16 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR10, transactivation domain B112 and the nuclear localization signal (NLS) SV40. In addition, was FunsTF16 condon optimised to A. niger.

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)), and it is the DNA binding domain interacting with LexO that is used in FunsTF16. HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF16 carried mutant number 10 of HbaR, which had the following mutations: A45S, L64I, A86G, A88Y, Y96A and L97G.

The transactivation domain B112 is from E. coli, which were experimentally proven to initiate transcription of a synthetic promoter in S. cerevisiae (Ottoz et. al (2014)). The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

The designed function of FunsTF16 is to be used as a transcription factor that can initiate transcription from the 6xLexO minimal promoter (BBa_K4129115). No functionality was shown.

FunsTF18 is a synthetic transcription factor (sTF) based on sensor of benzoic acid derivatives (sBAD), which is a sTF in S. cerevisiae (Castaño-Cerezo et. al (2020)). FunsTF18 differ from sBAD, because it has an nuclear localization signal (NLS) and is codon optimised to A. niger. FunsTF18 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR12, transactivation domain B112 and the nuclear localization signal (NLS) SV40.

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)), and it is the DNA binding domain interacting with LexO that is used in FunsTF16. HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF16 carried mutant 12 of HbaR, which had the following mutations: A45S, L64I, F85M, A86G, A88Y and Y96S.

The transactivation domain B112 is from E. coli, which were experimentally proven to initiate transcription of a synthetic promoter in S. cerevisiae (Ottoz et. al (2014)). The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF57 is a synthetic transcription factor (sTF). FunsTF57 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF57 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR3, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR3 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF57 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF57 contained mutant 3 of HbaR, which has the following mutations: A45V, L69A, G71K, E77M, F85G, A86G, E87G, A88M, Y96A, L97Y, N99T and A100V.

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF58 is a synthetic transcription factor (sTF). FunsTF58 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF58 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR4, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR4 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF58 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF58 carried mutant 4 of HbaR, which had the following mutations: A45V, L69A, G71K, E77W, F85G, A86G, E87G, A88G, A89P, Y96A, L97Y, A98M, N99T, A100V, and V142Q.

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF58 did not show functionality.

FunsTF59 is a synthetic transcription factor (sTF). FunsTF59 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF59 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR5, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR5 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF59 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF59 carried mutant 5 of HbaR, which had the following mutations: A45V, L69A, G71K, I76V, E77M, M79L, A86G, E87G, A88M, Y89A, L97P, N99T, A100V, V145Y and K148Y.

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF59 did not show functionality.

FunsTF61 is a synthetic transcription factor (sTF). FunsTF61 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF61 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR7, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR7 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF61 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF61 carried mutant 7 of HbaR, which had the following mutations: A45V, L69A, G71K, E77M, Y96A, L97F, N99T, A100V and V145M.

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

The intented function was not proven.

FunsTF62 is a synthetic transcription factor (sTF). FunsTF62 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF62 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR8, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR8 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF62 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF62 carried mutant 8 of HbaR, which had the following mutations: A45V, L69A, G71K, E77A, A86G, E89G, A90G, A91P, Y96A, L97Y, A98S, N99T, A100V and V145Y.

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF63 is a synthetic transcription factor (sTF). FunsTF63 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF63 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR9, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR9 is a longer version linker (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF63 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF63 carried mutant 9 of HbaR, which had the following mutations: L64V, F85T, A88G, A90Y, Y96H and L97G

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF65 is a synthetic transcription factor (sTF). FunsTF65 should initiate the transcription through the 6xLexO minimal promoter. This sTF is the sensing part of the biosensor.

FunsTF65 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR11, transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR11 is a longer version linker (Ottoz et. al (2014)) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)). FunsTF65 was codon optimised to A. niger .

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, namely LexO sites (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF63 carried mutant 11 of HbaR, which had the following mutations: A45S, L64I, F85M, A86G, A90Y and Y96A

Viral Protein 16 (VP16) from Herpes simplex virus type 1 is a transcription factor with a transactivation domain that recruits RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

FunsTF70 is a synthetic transcription factor (sTF). FunsTF70 should function as a transcription factor that can initiate transcription of the 6xLexO minimal promoter (BBa_K4129115). This sTF will be the sensing part of the biosensor.

FunsTF70 is a fusion protein consisting of the DNA-binding domain LexA, the ligand sensing domain HbaR16, the transactivation domain VP16 and the nuclear localization signal (NLS) SV40. The linker between LexA and HbaR16 was a longer version (Ottoz et. al (2014) compared to sBAD, which was the reference sTF (Castaño-Cerezo et. al (2020)).

LexA is a repressor that regulates the SOS response in E. coli (Radman. 1975). LexA binds to a specific DNA motif, LexO (Erill. et al (2003)). HbaR is a transcription factor from Rhodopseudomonas palustris that initiates transcription in the presence of benzoic acid (Egland. et al (2000)) or in the presence of benzoic acid derivatives (Castaño-Cerezo et. al (2020)). We created 16 mutants of HbaR and FunsTF70 carried mutant 16 of HbaR, which had the following mutations: L64I, F85H, A86G, A90Y and L97G.

Viral Protein 16 (VP16) from herpes simplex virus type 1 is a transcription factor that uses a transactivation domain to recruit the RNA polymerase II (Hirai et al. (2010)).The NLS SV40 is a small peptide sequence of PKKKRKV that enables transport of the protein to the nucleus (Garcia-Bustos et. al (1991)).

The promoter gpdA from A. nidulans was minimised to the minimum amount of nucleotides (180 bp) for transcription and was named Pmin. The transcription from Pmin was minor, but can still assemble the RNA polymerase if that process is initiated by a transcription factor. This system was used to make a tetracycline inducible system, TetOn, (Wanka et. al (2016)).

LexO is the name of the binding motif for LexA. LexA is the main regulator for the SOS response in E. coli (Radman (1975)). It has been shown in A. niger, that repeating LexO sites increase binding affinity and expression of a minimal promoter. The consensus number of LexO binding sites are six, 6xLexO (Rantasalo et. al (2018)).

The part is meant to be used as a test device to investigate the activity of the scFDH1 promoter in A. niger. The promoter regulates expression of mCherry with a C-terminal secretion signal peptide. The part was obtained by inserting the scFDH1 promoter (BBa_K4129000) in place of the prokaryotic promoter of BBa_K3046009 by USER cloning.

The part is meant to be used as a test device to investigate the activity of the scFDH2 promoter in A. niger. The promoter regulates expression of mCherry with a C-terminal secretion signal peptide. The part was obtained by inserting the scFDH2 promoter (BBa_K4129001) in place of the prokaryotic promoter of BBa_K3046009 by USER cloning.

The part is meant to be used as a test device to investigate the activity of the anFDH promoter in Aspergillus niger. The promoter regulates expression of mCherry with a C-terminal secretion signal peptide. The part was obtained by inserting the anFDH promoter (BBa_K4129002) in place of the prokaryotic promoter of BBa_K3046009 by USER cloning.
Since the anFDH promoter is responsive to furfural, this construct could represent the first furfural biosensor ever built.

Expression cassette made of a strong promoter PgpdA (BBa_K4129024) and terminator TtrpC (BBa_K678036). Gene expressed is arz7774 (BBa_K4129007) an aldehyde dehydrogenase from Amorphotheca resinae.

Expression cassette made of a strong promoter PgpdA (BBa_K4129024) and terminator TtrpC (BBa_K678036). Gene expressed is fucO (BBa_K4129008) a lactaldehyde oxidoreductase from E coli.

Expression cassette made of a strong promoter PgpdA (BBa_K4129024) and terminator TtrpC (BBa_K678036). Gene expressed is hmfH (BBa_K4129009) an Oxidoreductase from Cupriavidus basilensis.

This is a 2 kb homology arm, facilitating homologous recombination to genomically insert a cassette using CRISPR Cas9. This sequence has homology upstream of niger insertion site 1.

This is a 2 kb homology arm, facilitating homologous recombination to genomically insert a cassette using CRISPR Cas9. This sequence has homology downstream of niger insertion site 1.

An integratable gene expression cassette with arz7774 with pyrG as selection in A. niger integration site 1.

An integratable gene expression cassette with fucO with pyrG as selection in A. niger integration site 1.

An integratable gene expression cassette with hmfH with pyrG as selection in A. niger integration site 1.

An integratable gene expression cassette with arz7774 with pyrG as selection in A. niger integration site 1

An integratable gene expression cassette with fucO with pyrG as selection in A. niger integration site 1

An integratable gene expression cassette with hmfH with pyrG as selection in A. niger integration site 1

Transient expression platform for A. niger

arz7774 in transient expression vector

fucO in transient expression vector

hmfH in transient expression vector

Will make double stranded break at insertion site #1 in A. niger

Will make double stranded break at insertion site #1 in A. niger

Will make double stranded break at insertion site #3 in A. niger

Plasmid to transiently express BBa_K4129004 in A. niger

Plasmid to transiently express BBa_K4129005 in A. niger

Plasmid to transiently express BBa_K4129006 in A. niger

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF01. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF02. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF04. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF65. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF70. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.