In the scientific community it is important that results can be replicated. In order to achieve this our lab books and protocols are shown below as PDF. So even you can replicate our work.

Introduction

Find the relevant lab book, and download it by clicking the link.

Gene expression in A. niger

Lab book 1 - Cas9 and donor plasmid assembly
Lab book 2 - asembly of transient expression plasmid

Furfural converting enzymes

Lab book 1 -A. niger transformation with plasmids containing furfural converting enzymes
Lab book 2 - Assembly of plasmids with furfural containing enzymes
Lab book 3 - Cultivation of A. niger mutants
Lab book 4 - HPLC analysis
Lab book 5 - Testing the germination of A. niger mutant spores

Fungal Promoters

Lab book 1 - New fungal promoter testing
Lab book 2 - Promoter insertion strategy

Fungal Synthetic Transcription Factors

Lab book 1 - A. niger integration
Lab book 2 - FunsTF assembly
Lab book 3 - FunsTF characterization
Lab book 4 - FunsTF mutagenesis

Collaborations

Lab book 1 - Interlabs study 2022
Lab book 2 - Synthetic transcription factor - TU Delft

Modeling notebook

Dry lab modeling notebook Dry lab book - Modeling