Measurement

Synthetic Biology needs great measurement approaches for characterizing parts, and efficient new methods for characterizing many parts at once. Describe your measurement approaches on this page.

The specific measuring procedure of our sensor is 1. De-oxygen; 2. Add testing solution (containing GOx); 3. Apply CV (cyclic voltammetry); 4. Determine current response at the inversion potential (-0.8 V). The expected sensitivity and limit of detection (LOD) of this biosensor are 326 ± 5 mA M−1 cm−2 and 4.7 µM respectively. The testing samples include drinking (mineral) water, milk, plasma, natural water, etc. Also, selectivity, repeatability, and reproducibility should be examined. For selectivity, we have to test the samples with common interfering ions like uric acid, ascorbic acid, Cl−, SO42−, K+, Ca2+, and CN-. For repeatability, it means the electrochemical response of a single electrode does not change significantly during consecutive runs. To test reproducibility, we have to make sure that the results of electrochemical responses of seven different electrodes fabricated in the same batch are similar.