Basic laboratory safety
In most experiments, we used two safe laboratory strains, Escherichia coli（E.coli）DH5alpha, and E.coli BL21. In order to improve the safety of our experiments, we have developed a laboratory safety rulebook.
In most experiments, we used two safe laboratory strains, Escherichia coli（E.coli）DH5alpha, and E.coli BL21. In order to improve the safety of our experiments, we have developed a laboratory safety rulebook.
Laboratory safety rulebook
1. Wear slippers are not allowed to enter the laboratory.
2. Don't wear gloves to open the door.
3. You must always wear a mask and a white coat.
4. You can't eat and drink water in the laboratory and store food in the refrigerator.
5. No form of playfulness is allowed.
6. If you see the team members behave dangerously, please point out to them.
7. Unauthorized experiments are not allowed without care.
8. Keep the laboratory area clean and tidy and standardize the use of laboratory equipment.
9. Clean up the experimental waste after the experiment.
Biosafety for specific projects
Our experiment need to perform experimental operation in the ultra-clean table, also had the use of the toxic substance ethidium bromide in nucleic acid electrophoresis. We require team members: to spray alcohol with both hands to disinfect it when operating in the ultra-clean table, then put it into the ultra-clean table to dry the alcohol before the next operation; cultivate Escherichia coli When you don't touch the lower part of the test tube plug, put the test tube plug on the alcohol lamp for disinfection before and after use, and use the alcohol lamp when opening the LB medium bottle cap; when performing nucleic acid electrophoresis, you should wear double gloves and the outer layer is plastic gloves to avoid contact with ethidium bromide.
All the genes in our experiment were synthesized directly through DNA synthesis. At this stage of the experiment, the strain has never been taken out of the laboratory. The entire sensor system we expect to design will work in the wild environment, but our strains will only be kept in the sensor system and will not be released into the field, so as to ensure that it does not cause animal, plant and environmental safety problems. We will also carry out a strict sterilization process after field work is completed to ensure maximum safety.
