Introduction
Reaching out to specialists and simultaneously presenting the initiative to the general public are always key components of integrated human practice. Our research, in particular, needs public awareness because everyone is at danger from the possible health risks of bisphenol A because its widespread manufacture has already negatively impacted our way of life. In order to help more people understand and get familiar with BPA, our team made an effort to engage with the general public. To reflect and make our project better, we also spent a lot of time talking to and interviewing experts (specifically on biosensors and chemical analysis).
Initial model
In the first model of our design, our team was inspired by a research project and a previous iGEM team to build a modified enzyme to degrade bisphenol A to another, harmless substance.
https://annalsmicrobiology.biomedcentral.com/articles/10.1007/s13213-013-0649-2
https://2011.igem.org/Team:Bielefeld-Germany/Project/Background/BPA
However, after communicating with other iGEM teams doing similar projects that focus on environmental pollutants, our team concluded that building a modified enzyme to degrade BPA was not the best course of action.
Unlike other similar teams whose pollutants is only present in the environment, bisphenol A is everywhere --- from rivers to plastic bottles, even in feeding bottle of infants. The deteriorated substance could not be allowed to remain in the food or containers. Furthermore, based on the quantity of BPA, the degradation process would take a very long time to finish.
Thus, we all agreed that the aim of our project is to design a biosensor to detect bisphenol A.
Public survey and education
So that after the team had settled to build a biosensor, BFSU-ICUnited conducted a public survey from July 26 to September 11.
Aiming to
- Educating the public on the general facts & the health risks of BPA
- Ascertain the general opinion on knowledge of BPA
- Mostly importantly, to get a understanding of the stakeholders in our project so that our design suitable to the needs
With a response from nearly 950 people (n=931), we obtained the following findings and successfully popularized basic information about the toxicity of bisphenol A to the respondents.
Our pole is aimed to reach to people of all age since everyone is a stakeholder concerning bpa (for we all use plastic materials so we are exposed to it). And based on our results, it did.
Have you heard of BPA before ?
From our findings, it can be seen that 27.93% (260 people) have heard of bpa before, while 72.07% (671 people) never know what bisphenol A is. Among those who have heard of bisphenol A, 8.46% (22 people) are very aware of the harm of bisphenol A to the human body, 67.31% (175 people) know a little, and 24.23% (63people) have no awareness. Through the above data, we know that nearly three-quarters of the respondents have never heard of bisphenol A, and the majority of those who have heard of bisphenol A are slightly or not, so we have concluded that the public lacked understanding of bisphenol A.
Do you also use products containing BPA in your daily life ?
How often do you use products containing BPA ?
Through the above survey data, we know that many people use products containing BPA more or less in their daily lives, but few people know about its toxicity and its harm to the human body. Through this questionnaire, we have also popularized the basic knowledge of BPA to the respondents. At the end of the questionnaire, we asked the respondents if they had a preliminary understanding of BPA through this questionnaire. 93.88% (874 people) answered "yes" and 6.12% (57 people) answered "no". The number increased a 98 percent and thus it can be safe to say that our attempt is successful.
Moreover, this poll had shown us that everyone is a potential user of BPA detection products since they themselves feel that their health is threatened after learning BPA's potential health risks. This pole has shaped our project significantly.
Second Model
Thus, we decided to design a biosensor that is portal, convenient, cheap, and most importantly, suitable for family use, for everyone. Only this way the design cycle was closed.
Looking into biosensor projects, we have found that many are based on GFP (green fluorescent protein), so our team was encouraged to construct a biosensor based on GFP. The corresponding result would be to observe flowing in the presence of BPA. The imperfection in this model lies with the price of fluorescence microscopes --- the average cost up to five thousand dollars. This obviously didn't satisfy our aim that the biosensor has to be suitable for family use, not only in test centers.
Third Model
After consulting experts in BCPCA (Beijing Center for Physical & Chemical Analysis), they suggested to use electrochemical enzymed based biosensor to detect BPA. And electrochemical biosensors "can recognize the electrical signal from the detected substance and test the concentration of the detected substance through signal strength. It has high sensitivity, accuracy, and wide applicability."
Final Model
In the third model, the tyrosinase is located inside the E.coli, which involves transmembrane transport. However, transport between membranes significantly reduces the sensitivity of the biosensor. And the concentration of BPA in real life is often low --- about 1 µg/L [1], thus we require a highly sensitive biosensor to detect BPA.
According to and thank advice from Professor Hou, the team considered and evaluated the LOD values of different systems using tyrosinase and concluded that using cell surface display in the biosensor is the most sensitive design.
This is the final model in our project.
Educational Cup design
Despite the positive results of our educational poll, many people are still unaware of BPA (as can be seen by the initial 72 percent completely unaware of BPA). The public needs to be made aware of the risks that BPA poses to their health. Since everyone is a stakeholder on the matter of health matters threatened by BPA, BFSU-ICUnited has made an effort to inform the public of the substance. We have created a porcelain cup to warn the general public of its dangers.
The health advantages of drinking water and a list of BPA's health risks are printed on the cup. Our team hopes that using this cup will serve as a reminder to drink water and to avoid BPA as much as possible. Since the cup is made of porcelain rather than plastic, consumers are completely protected from BPA.
Hopes for the future
Initially, we debated whether to produce a replacement for the material after the team decided to base the project's design on bisphenol A. After considering its production number every year, the team chose to to produce a detection machine.
However, neither BPA degradation nor a biosensor used for detection is a permanent fix for its dangers. Therefore, we think it will be essential for a replacement material to be developed and put on the market at some point in the future. Being a responsible team deeply concerned everyone's ( people use plastic materials containing BPA, that is nearly everyone) health conditions, BFSU-ICUnited looked into the possibility of creating a substitute for BPA.
Mr. Tian and one of our group members
When Mr. Tian from LanZhou University dropped by, he showed a keen interest in our project. We consulted him for suggestions on creating a BPA replacement.
He maintained that creating a replacement is crucial. Same as us, Mr.Tian believed that it is impossible to replace it completely all at once based on production statistics (1 million pounds per year [2]), for it would be too much work for us to do. The more likely method is to try to introduce the replacement material to the higher-end market first, where consumers and manufacturers can accept the initially high cost of plastic materials. Then, as time goes on and the cost of manufacturing the replacement material drops, BPA can be completely replaced.
We received the same answer after inquiring about this matter with a group of doctors, who are very familiar with the possible health hazards of BPA. They've also pointed out that price is one of the factors that have to be put in mind since common buyers of plastic products won't afford any higher price. So that we are a long way from developing a possible substitution for BPA to be used in products in commercial companies.
Hopefully, someday in the future, people living under the penetration of plastic products into their lives, after being offered detection and degradation plans, will finally have a replacement of BPA in plastic products and live a life free of it.
1- https://www.factsaboutbpa.org/bpa-overview/environment/#:~:
text=When%20biomonitoring%20studies%20have%20detected%20BPA%20in%20streams,
in%2040%2C000%20gallons%20or%20150%2C000%20liters%20of%20water.
text=When%20biomonitoring%20studies%20have%20detected%20BPA%20in%20streams,
in%2040%2C000%20gallons%20or%20150%2C000%20liters%20of%20water.
2- https://www.epa.gov/assessing-and-managing-chemicals-under-tsca/risk-management-bisphenol-bpa