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Protocols

    Overview

    To make sure everybody on our large team is on the same page regarding the way the experiments are performed, we wrote protocols for every single experimental step. The protocols helped us to coordinate our lab subgroup with nearly 20 members. They provided information on needed substances, devices and experimental steps. This reduced misunderstandings and errors during the execution of experiments and enabled our team to work precisely and independently.

    Buffers and Solutions

    Below are the protocols for buffers and solutions that we have used in our daily laboratory work.


    Autoinduction Medium
    HEPES Buffer
    IPTG Solution
    Kanamycin Stock Solution
    LB Medium
    Nickel Sulphate Solution
    PBS Buffer
    TB Medium
    TFB Solution
    Tris-HCl Buffer
    Experiments

    These protocols provide a guide to record the experiments we have used for our protein expression, purification and phosphate detection methods.


    Agarose Gel Electrophoresis
    Amicon Buffer Exchange
    Bacterial Expression
    BCA Assay
    Bradford Assay
    Cell Disruption with Ultrasound
    Check-PCR
    Colony PCR
    Competent Cells
    Cryoculture
    Extraction of Polyphosphate from Saccharomyces cerevisiae
    Gibson Assembly
    Illumination Process
    Immobilization
    Phosfinity Assay
    Plasmid DNA Purification
    Production of Polyphosphate-rich Saccharomyces cerevisiae Cells
    Purification Using a His-Tag
    Two-step PCR for Site-directed Mutagenesis (QuikChange)
    SDS-PAGE
    Transformation

    iGEM Aachen 2022

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