Overview

As part of the synthetic biology community, it is also in our interest to make biology more accessible and easier to engineer. Genetic parts that can be mixed and matched are crucial to build new biological systems and devices. This is why we listed below the parts we used in our project to engineer our fusion proteins. Additonally, we documented and registered them in the iGEM Parts Registry to maintain the open knowledge database and in the hope that these parts could help other teams or labs who aspire to build a switchable protein in the future. For each protein we used, we have created a factsheet where you can find all the relevant facts. Below you will find a table with all our factsheets.

Genetic Parts

Name	Type	Description	Designer	Length
BBa_K4138000	Phosphate binding protein	Phosphate binding protein (2ABH) acts as phosphotransferase from E.coli	Carlos Classen	963 bp
BBa_K4138001	Photosensitizer	Photosensitizing protein (SOPP3) for protein disruption	Carlos Classen	378 bp
BBa_K4138002	Photoreceptor	Photoreceptor (VVD) from Neurospora crassa dimerizes under influence of blue light	Carlos Classen	447 bp
BBa_K4138003	Immunity protein	Homodimer (CMI) that uses phosphate ions as a ligand	Carlos Classen	294 bp
BBa_K4138004	Fusion construct	Fusion construct of photosensitizer and phosphate binding protein	Carlos Classen	1416 bp
BBa_K4138005	Fusion protein	Fusion protein of VVD and CMI combined with a linker	Carlos Classen	777 bp
BBa_K4138006	Linker	Serine-Glycine Linker for VVD-CMI	Carlos Classen	36 bp
BBa_K4138007	Spacer	Serine-Glycine Spacer for SOPP3-2ABH	Carlos Classen	72 bp
BBa_K4138008	2ABH forward primer	primer for Gibson assembly for the 2ABH	Carlos Classen	46 bp
BBa_K4138009	2ABH backward primer	primer for Gibson assembly for the 2ABH	Carlos Classen	44 bp
BBa_K4138010	Vector forward primer	Primer for Gibson assembly for the vector pET-28a(+)	Carlos Classen	29 bp
BBa_K4138011	Vector backward primer	Primer for the Gibson assembly for the vector pET-28a(+)	Carlos Classen	25 bp

Factsheets

Here you can find the factsheets of the used proteins of our project.