Global energy exhaustion has been a serious issue all over the world. Until now, the world has made many efforts on developing new energy sources and technologies, but many of refacing various problems, such as expensive raw materials and the inability of mass-production. Butyl butyrate is a new class of biofuels with high performance. We engineered Clostridium tyrobutyricum to produce both butyric acid and butanol at the same time, and esterify it to form butyl butyrate under the catalysis of lipase. The aldehyde alcohol dehydrogenase AdhE2 gene were introduced into C. tyrobutyricum to convert butyryl-CoA to butanol, and the expression of Thl and Hbd were also strengthened at the same time to improve the yield of butyryl-CoA. Using discarded shrimp and crab shells as a kind of nutrient source, the green production process of butyl butyrate from kitchen waste to biofuels is realized.