ABSTRACT
ABSTRACT The long-existing problem of soil salinization has worsened in recent years. As a novel class of microbial feed additives, Clostridium tyrobutyricum has great development prospects. We wish to tackle the worsening issue while contributing to the reduction of antibiotic use. We improved the oxygen tolerance of C. tyrobutyricum during feed additive production and after emission into the environment, through heterologous expression of DNA protection proteins Dps together with pyruvate dehydrogenase AceE, to increase the yield of its additives and develop its application as an acid fertilizer used for the solutions for soil salinization. We wish our project could help reduce the overusing of antibiotics and meanwhile further to improve the soil salinization problems, achieving dual healing for gut and soil.