Experiments

Basic Operations

Inoculation

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Glycerol stock

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Waste liquid treatment

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Autoclave

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Cloning

PCR, electrophoresis, gel extraction

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zymo sol+boil

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zymolyase processing

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SLiCE

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Golden Gate Assembly

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E. coli transformation

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Yeast transformation (LiOAc method)

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Plasmid extraction

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Gibson Assembly

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Medium and Gel Composition

LB medium

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YPD medium

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SC-U medium agar

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Agar gel for electrophoresis

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