Team:USTC
SCUT
Abstract
We have totally held three online meeting during our cooperation, through which we fully exchanged our opinions and brainstormed to figure out solutions of the problems we faced. Both sides of us benefited a lot by interflowing with each other. As for SCUT, we shared our idea of moving the MVA pathway into the peroxisome with them, which helped them reduce the cellular metabolic stress. Additionally, Yarrowia lipolytica with ERG19 and PTS1(peroxisomal targeting sequence) was sent to them, since we wanted to finish the MVA pathway together. Those assistance promoted their project and created many positive results.In turn, they helped us release the worry about safety of manufactory pollution by giving a sequence of light-induced transcription factor to us. Also, their IUP pathway showed us a possible way to improve our project.
We have totally held three online meeting during our cooperation, through which both sides of us benefited a lot. As for SCUT, our idea of moving the MVA pathway into the peroxisome and our Yarrowia lipolytica with ERG19 and PTS1 were shared with them, since we did wish to finish the MVA pathway together. Those assistance promoted their project and created many positive results. In turn, they helped us release the safety worry of manufactory pollution by giving a sequence of light-induced transcription factor to us. Their IUP pathway also showed us a possible way to improve our project in the future.
Main
Our first online meeting with SCUT-China was held on August 8th , through which we fully exchanged our opinions and shared our experience about our experiments.
This meeting inspired both of us to introduce our projects to more people, thus we post particles about our works on the two team’s WeChat official accounts. Parts of the two particles are shown below.
In later experiment, we held two online meeting to solve the problems we met and rounded out our projects.
Assistance from SCUT-China
Before communicating with them, although we got a seemly responsible way to biosynthesize borneol with high efficiency, we were disconcerted by the potential risk of manufactory pollution of yeast. Thanks to their light-induced transcription factor, we soon successfully designed a light-induced suicide-switch system to solve the safety problem.
Additionally, though we had already got a sight of the IUP pathway before, their use of this pathway showed us a possible way to improve our project in the future.
Assistance to SCUT
After a brief introduction, we soon found a common ground that both sides of us had chose yeast as our chassis, and planned to produce terpenoids through the MVA pathway. However, their project was facing some obstacles. That is, although they utilized the most prolific promoter, PDAS2, it influenced the growth of the cells and would finally lead to the reduce of the productivity. Our team had solved this problem by moving the MVA pathway into the peroxisome. So we shared our idea to them and also sent them our Yarrowia lipolytica with ERG19 and PTS1(peroxisomal targeting sequence), for the aim to finish the MVA pathway together.
Later, our team member went to Guangzhou on August 13th to have a deeper conversation with them.
After hearing our advice, they re-constructed the metabolic pathway of the yeast and use lsoamyl enol as carbon source. Finally they managed to added five enzymes to the pathway and got positive result.
USTC-Software
Abstract
A good alliance is conducive to the long-term development of both sides. Besides SCUT, we are closely associated with USTC-Software, who makes use of AI to design software while we are doing experiments. We have had a lot of meaningful communication throughout the project. Our cooperation with USTC-software ran through the whole process. We have held two joint group meetings face to face and many online detailed discussions.
Assistance towards USTC-Software
Because most of their players aren’t major in biology. We helped them with professional knowledge to shrink research direction to a small scale. Their initial idea was to develop a platform to analyze and predict protein interactions by using transcriptomes mechanism. Discussions between us helped them to further clarify the feasibility and significance of the idea and understand the needs of selecting proper molecule structure in laboratory. After the discussion, they made appropriate adjustments to our direction and optimized the design of our software and add some practical small functions on the interface.
At the same time, we provided a mass of practical results and resource for them to set up their ideal database website. For example, the website of KEGG and brenda is excellent model for them to distinguish the pathway with greater efficiency. We also card almost majority of enzyme catalysis reaction pattern and accomplished the number statistic of different reaction, finally draw a table below. More helps such as some common properties of biomolecules and chemical skills also offer the principle support to the operation of our software.
Assistance from Partner
Because of our aim of project, which is to select the most productive enzyme after transfection, we tested our manual screening consequence on the website built by USTC-Software.Finally we confirmed our choice and retrieved several similar enzymes with equal function. Deeper comprehension into the type of enzymes facilitated our adjustments of selection tag and methods of cultivating yeast.
External Visiting
Mutual visiting to the factory facilitated both of us, we consulted how to transform the ketone into amine to accomplish our medicine carrier while USTC-Software asked its distinguished biological technical experts for their data as samples to examine their analysis platform. Industrialization experience was valuable aid for our optimization process from different dimensions.
