Parts | Uni-Hamburg

Our SPEAR System consists of two split ribozyme halves, two guide RNAs, one RBS and one reporter. The two parts are expressed by two different promoters, as the similarity in the sequence when using the same promoter prevents it from being synthesized. We put the single parts of our detection system into the registry singularly to facilitate usage by others. Especially if you are using our software to create your own split ribozyme system it is helpful to see the singular parts and how they were put together by us. In the following you can find our part pages from the registry for split ribozyme halves and ribosome binding site, the guideRNAs and the composite parts that are built up by them.

Split ribozyme halves and ribosome binding site

name	type	description	designer	length (bp)
BBa_4472999	device	split ribozyme half 1	Gambill et al.	70
BBa_4472998	device	split ribozyme half 2	Gambill et al.	410
BBa_4472997	RBS	RBS 1	Gambill et al.	8

guide RNAs

name	type	description	designer	length (bp)
BBa_4472996	device	guide RNA 1 detecting kanamycin resistance	Stine Behrmann	82
BBa_4472995	device	guide RNA 2 detecting kanamycin resistance	Stine Behrmann	82
BBa_4472994	device	guide RNA 1 detecting stable endogenous E.coli mRNA hcat	Stine Behrmann	82
BBa_4472993	device	guide RNA 2 detecting stable endogenous E.coli mRNA hcat	Stine Behrmann	82

composite parts: functional split ribozyme units

name	type	description	designer	length
BBa_K4472991	composite	detects hcat and expresses GFP	Stine Behrmann	1700
BBa_K4472990	composite	detects kanR and expresses GFP	Stine Behrmann	1700
BBa_K4472989	composite	detects kanR and expresses eforRED	Stine Behrmann	1661
BBa_K4472988	composite	detects hcat and expresses eforRED	Stine Behrmann	1661