Part Collection

We designed several split ribozyme combinations with different guideRNAs and reporters.

Our split ribozymes are designed to detect kanamycin resistance of pSB1K3 or the stable endogenous E. coli mRNA hcat1, that codes for a putative 3-phenylpropionate transporter2. We combined these split ribozymes with the fluorescent protein GFP and the chromoprotein eforRED using GoldenGate Assembly and summarized the parts in this part collection.

name

type

description

designer

length

BBa_K4472991

composite

detects hcat and expresses GFP

Stine Behrmann

1700

BBa_K4472990

composite

detects kanR and expresses GFP

Stine Behrmann

1700

BBa_K4472989

composite

detects kanR and expresses eforRED

Stine Behrmann

1661

BBa_K4472988

composite

detects hcat and expresses eforRED

Stine Behrmann

1661


[1] Zhou, K., Zhou, L., Lim, Q.'. et al. Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR. BMC Molecular Biol 12, 18 (2011). https://doi.org/10.1186/1471-2199-12-18
[2] https://biocyc.org/gene?orgid=ECOLI&id=G7330