| Part | Part Name | Description |
| BBa_K4357000 | XeR (xenorhodopsin derived from Nanosalina spp.) | The light-driven inward proton pump XeR is derived from xenorhodopsin in Nanosalina spp. It is a seven-transmembrane alpha-helix protein that functions with its cofactor called retinal(chromophore). When there is a light source applied to the protein, the conformational change of the retinol will induce inward proton pumping of bacteria and generate an electrochemical proton gradient across the membrane. |
| BBa_K4357001 | Pasr-XeR-mCherry-pSB1C3 | In the direction for our gene expression, there is an asr promotor (BBa_K1231000) which is the pH-responsive promoter native to E. coli, inducing transcription in acidic conditions (~pH 5.5), RBS (BBa_B0034) which is the ribosome binding site, XeR gene which is our target gene, controlling the expression of inward H proton channel, HRV 3C site which is the linker between the target gene and reporter gene, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell). |
| BBa_K4357006 | J23100-mCherry-pSB1C3 | In the direction for our gene expression there is a promotor (BBa_J23100) from Constitutive promoter family member which has the strongest strength among BBa series of promoters in the Anderson promoter library, RBS (BBa_B0034) which is the ribosome binding site, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell). |
| BBa_K4357003 | mCherry+his tag | This is a mCherry fluorecent signal protein with a complement with a 6xhis tag. This part is used for tracing the target protein expression level or examine the function of a promoter |
| BBa_K4357004 | Pasr-mCherry-pSB1C3 | In the direction for our gene expression there is an asr promotor (BBa_K1231000) which is the pH-responsive promoter native to E. coli, inducing transcription in acidic conditions (~pH 5.5), RBS (BBa_B0034) which is the ribosome binding site,mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell). |
| BBa_K4357005 | J23100-XeR-mCherry-pSB1C3 | In the direction for our gene expression there is a promoter (BBa_J23100) from Constitutive promoter family member which has the strongest strength among BBa series of promoters in the Anderson promoter library, RBS (BBa_B0034) which is the ribosome binding site, XeR gene which is our target gene, controlling the expression of inward H proton channel , HRV 3C site which is the linker between target gene and reporter gene, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell). |
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