Team Affairs:

• Assign work to each group leader and coordinate the cooperation between groups.
• Organize regular meetings between team members and team primary PI and instructors to promote the progress of the project.
• Project Design:
• Propose ideas, search data, and plan experiments with team members.
• Summarize and organize the primer list.

Wiki:

• Organize and coordinate team members to write wiki content.
• Check the wiki page details.

Modeling:

• Search related materials and discuss with team members how to achieve the modeling goals.
• Check and confirm final models.

Education:

• Set up stalls twice at our own university to introduce igem competition and our project and communicate with other students and teachers.
• Help team members to contact the person in charge for team preaching in high school.

Outreach:

• Co-organized the “HydroAqua iGEM League 2022” with PuiChing Macau.
• Participate in the G20 iGEM China Summit held by iBowu-China and SHSBNU.
• Participate in Freshwater Bioremediation Alliance held by JNU-China and GXU-China.

Videos:

• Lead team members to design the outline and core part of the promotion video.
• Prepare the PPT for the promotion video and be the person to present.

Secretary:

• Participate in writing and modifying news reports.
• Help purchase materials needed for human practice work.
• Coordinate team members to complete the judging form and check and summarize the main information.

Project Design:

• Verified the topic for the iGEM project: elaborate on ideas and design plasmids and primers for the project. Propose the experiment plan to the team
• Team logo design.
• Name and design the backgound of the project.

Team Affairs:

• Communicate with the captain and other group leaders.
• Coordinate with faculty and university for team affairs.
• Supervise teamwork progress.
• Film team members’ pictures.
• Generate and review proposals for the project.

Modeling:

• Data searching and exchanging ideas with the dry lab members in order to decide specific model. Providing experimental results from the wet lab and determining the initial condition for the model.
• Contact with adivisor.

Wetlab:

• Prepare competent cells (DH5 Alpha).
• Prepare LB, M9 medium, and chemical reagents.
• Construct plasmids with the target gene for E.coli transformation by using PCR, gel electrophoresis, gel purification, miniprep, Gibson assembly, and transformation.
• Fluorescent signal measurement as Pasr promotor function assay. Measure the OD600 value, fluorescent signal, and pH changes of the medium as XeR function assay. Perform PCR and mini prep for vector backbone and XeR gene.
• Determination of gene expression by RNA extraction and Real-time PCR. Confocal image shooting for engineered E. coli cells.
• Data analysis for real-time PCR and functional assays.

Human Practice:

• Draft the outline of the whole human practice work.
• Contact and visit the Macau DSPA Waste Water Treatment Plant.
• Participate and draw the comic book event organized by Tec-Chihuahua.
• Design our coloring brochure.
• Prticipate in PPT making and content sharing on meetups.
• Co-organized the “HydroAqua iGEM League 2022” with PuiChing Macau.
• Participate in G20 iGEM China Summit held by iBowu-China and SHSBNU.
• Participate in Freshwater Bioremediation Alliance held by JNU-China and GXU-China.
• Participate in the filming of MetaThess collaborating video.
• Plan the invitation email of the corporation partnership.

Outreach:

• Contact Puiching_Macau for plasmids exchanging and events holding.
• Contact with Jiangnan-China for plasmids exchanging.
• Contact local primary school for preparing the educational events.
• Contact local government.

Wiki:

• Writing the wiki content.
• Design wiki foramt.
• Drawing background images and icons.
• Presentation Video:Prepare for presentation video PPT and scripts.
• Check and correct wiki mistakes.

Promotion Video

• Participated in modifying the manuscript of the promotion video.
• Material preparation, drawing the scene.
• Shoot stop-motion animation.

Secretary:

• Write judging form.
• Check and modify the final version of judging form.
• Propose outreach budget.

• Primer design, culture media preparation and pH adjustment, PCR amplification, DNA gel running, DNA gel purification, Colony picking, plate drawing, bacterial culture, concentration measurement, and fluorescent measurement.

• At the beginning, participates in the lab materials and tools preparation. Fluorescent signal measurement as Pasr promotor function assay. Pre-experiment for DH5 alpha E. coli strain (OD measurement and fluorescent signaling test using RFP-engineered E. coli cells).
• For the iGEM publicity, help advertise the iGEM program, and show many posters and activities to my friends during this time.
• Website making, collating the experiment protocol.

• Prepare competent cells (DH5 Alpha).
• Prepare LB, M9 medium, and chemical reagents.
• Construct plasmids with the target gene for E. coli transformation by using PCR, gel electrophoresis, gel purification, Gibson assembly, and transformation.
• Fluorescent signal measurement as the Pasr promotor function assay.
• Measure the OD600 value, fluorescent signal, and pH changes of the medium as the XeR function assay. Perform PCR for vector backbone and XeR gene.
• Determination of gene expression by RNA extraction and Real-time PCR. Confocal image shooting for engineered E. coli cells. Data analysis.
• Lab note organization.

• Prepare competent cells (DH5 Alpha).
• Prepare LB, M9 medium, and chemical reagents. Construct plasmids with the target gene for E.coli transformation by using PCR, gel electrophoresis, gel purification, midi prep, Gibson assembly, and transformation.
• Fluorescent signal measurement as Pasr promotor function assay. Measure the OD600 value, fluorescent signal, and pH changes of the medium as XeR function assay.
• Determination of gene expression by RNA extraction and Real-time PCR. Confocal image shooting for engineered E. coli cells.
• Data analysis for real-time PCR and functional assays.
• Lab note organization.

Wetlab:

• Helped with PCR amplification, gel electrophoresis, OD measurement of E.coli, and culture of E.coli in different pH.
• Human Practice: Helped with the proposal ppt.
• Involved in the pre-planning of video production.
• Wrote the script for the introduction video.
• Made posters for promotional activities.

Initial Idea Proposal:

• Proposed the project propose about help with water body acidification and proposed XeR gene to introduce an inward proton pump into E.coli to carry on the study

Wet lab:

• Measured the OD600 value as Pasr promoter function assay
• Confocal image shooting for engineered E. coli cells

IT:

• Prepared materials and images for the homepage.
• Collected required background photo materials.

• Help with PCR amlification, gel electrophoresis, and OD measurement of E.coli.
• Analyze graphs and write part content of proposal ppt.
• Fill in the information of wikipage

Secretary:

• Wrote news reports
• Wrote impact grant
• Wrote safety and security grant
• Wrote budget proposals
• Human Practice: Visit the Macau DSPA WasteWater Treatment Plant

Secretary:

• Wrote news reports
• Wrote impact grant
• Wrote safety and security grant
• Wrote title and abstract

Human practice:

• Purchased materials for outreach
• Participated in outreach

• Purchased information for outreach
• Participated in outreach

Team affairs:

• Communicate with the captain and other group leaders.
• Coordinate with faculty and university for team affairs.
• Name and design the project.
• Supervise teamwork progress.
• Film team photos and member photos.
• Collect team member pictures, introductions, and other information.
• Generate and review proposals for the project.

Wiki:

• Film wiki photos.
• Write wiki content.
• Ajust wiki structure.
• Edit wiki format.
• Check and correct wiki mistakes.

Promotion Video:

• Propose video idea.
• Direct the whole video.
• Write and modify the script.
• Drawing shooting board materials.
• Shoot video.
• Edit in chief of the video.

Integrated Human Practice:

• Main contact and the person in charge of all the human practice events, collaborations, and partnerships.
• Organize meetings of human practice.
• Draft the outline of the whole human practice work.
• Summarize and classify all the human practice work.

Education & Communication

• Participate in the outreach of the Macau Wastewater treatment plant.
• Organize campus promotional events.
• Contact the university for applying venue of campus promotional events.
• Design and modify posters.
• Organize design and printing of the coloring brochure.
• Shoot event photos and be the narrator of event content.
• Host an online class for middle school students in a remote area.
• Organize and be the main contactor of local primary school educational events.
• Contact of G20 iGEM China Summit and China igem online meetup.
• Participate in ppt making and content sharing in meetups.
• Run team’s official wechat account.

Collaborations

• Participate in the filming of MetaThess collaborating video.
• Plan the invitation email of the corporation partnership.

Partnerships:

• The contact of Freshwater Bioremediation Alliance.
• Participate in alliance meetups.
• One of the contacts of Puiching_Macau.
• Scheme the establishment of HydroAqua 2022 iGEM league.
• Organize and write the proposal of the online conference “HydroAqua Simulator”.
• Host “HydroAqua simulator” conference.
• One of the contacts of Jiangnan-China.

Secretary:

• Write judging form.
• Propose outreach budget.
• Help with material purchasing.

Team affairs:

• Summarize and calssify team documents.
• Supervise teamwork progress.
• Name and design the project.

Social Media management:

• Responsible for the operation of official instagram .
• Promote project on it; Liaised with other global teams on Instagram.
• Proposed, designed ideas for instagram page.

Human practice:

• Generated and reviewed drafts and final version for Proposals. Designed backdrop images for our team to use during virtual meetings.
• Proposed outreach budget plan for the iGEM project with Human Practice and IT members.
• Write meeting records.
• Deaign virtual background for our online confrence.

Public engagement:

• Participated in outreach to Macau DSPA Wastewater Plant Transplant.
• Participated and implemented collaborative activities with iGEM teams.
• Promoted projects in G20 iGEM China Summit held by iBowu-China and SHSBNU.
• Subtitles Editing to comic book event by iGEM Tec-Chihuahua and USAFA
• Participated in preparing conference manual for “HydroAqua Simulator” that is co-organised with the iGEM Pui_Ching team.

Promotion Video:

• Participated in proposing video ideas, preparing manuscript, material drawing, narration of script. Filming for slow-motion animation video.

Wiki:

• Compile contents for collaboration part of human practice.
• Contibute wiki photos.

Secretary:

• Write judging form.
• Help with material purchasing.
• Propose outreach budget.

• Participate in the activity, collecting the improved beer’s logo with Human practice members.
• Provide ideas for the project name and logo.
• Contact the local high schools.
• Contact partner companies.
• Contact other teams for collaboration and partnership.

• Held an activity to collect the improved logo.
• Video: Work with team members to make videos.
• Participated in modifying the manuscript of the promotion video, and looking for the scene part of the video to edit and modification.
• Poster: Participated in making, thinking, composing, editing, revising, and publishing posters with team members.

• Draw up sponsoring emails and communicate with sponsors.
• Help with other written work.

• Contact company for collaboration and partnership
• Design the cover of the brochure for educational and outreach events
• Add subtitles to the promotion video
• Write the wiki content

Team Affairs:

• Introduce members of the IT group and coordinate our work with the captain.

Wiki:

• Based on the structure constructed by team members, complement the content of the modeling part.

Modeling:

• Construct the mathematical model and provide some suggestions to optimize the details of the experiment, in turn, using the experimental data and previous papers.

Modeling:

• Analyzing the data from our research and other references.
• Brainstorming and developing ideas for modeling.
• Participating in mathematical model construction.
• Generating the diagram of the modeling.

Wetlab:

• Prepare competent cells (DH5 Alpha).
• Prepare LB, M9 medium, and chemical reagents.
• Construct plasmids with the target gene for E. coli transformation by using PCR, gel electrophoresis, gel purification, Gibson assembly, and transformation.
• Fluorescent signal measurement as the Pasr promotor function assay.
• Measure the OD600 value, fluorescent signal, and pH changes of the medium as the XeR function assay. Perform PCR for vector backbone and XeR gene.

Wiki:

• In charge of wiki.
• Write most of the code for structure of wiki.
• Design webpage appearance and interactivity.
• Collect materials for wiki.
• Proofreading wiki content.

• Together with team members, build the structure of the wiki website, mainly coding, and scriptwriting.
• Promote the team on display.

• As the convener of our team, Prof. Lee was responsible for administrative procedures and provided most of the suggestions on the experiments and human practices and assisted in project design.

• Prof Wong helped us a lot with our promotion and presentation video. He participated in the majority of our monthly meetings and gave us a lot of advice on our project.

• Prof Tam gave many suggestions on improving our function assay and modeling.

• A handsome and experienced technician from FHS faculty.
• He gave bunches of technique help in lab work.
• He entertained the wetlab members.

• A technician from the FHS faculty. She taught us how to use and set the confocal microscopy for E. coli fluorescent signal shooting