Attributions | Tec-Monterrey

Dry Lab

Wet Lab

Software &
Modeling

Human Practices

Educational
& Public
Engagement

Marketing

Finance &
Entrepre-
neurship

Strategic
Planning and
Projects

Wiki

Instructors
and advisors

Collaborators

Sections

Dry Lab

Wet Lab

Software &
Modeling

Human
Practices

Educational
& Public
Engagement

Marketing

Finance &
Entrepre-
neurship

Strategic
Planning and
Projects

Wiki

Instructors
and advisors

Collaborators

Dry Lab

The dry lab team came up with the project idea working for over a year to make a detailed investigation to back the project up in order to proceed with the laboratory tasks and performed the in silico analysis of the parts to ensure its viability.

Emilio Fabian and Ana Paola Morales were the principal contributors to the investigation of the different protocols and primer designs utilizing E. coli as the chassis. Sofia Goitia Favela, and Juan Antonio Alfaro were in charge of the background research by previous studies and other iGem teams and Juan Manuel Dominguez focused on the bacteriophages purification and infection protocols. The sRNAs were designed by the model team and the cloning strategies were developed by Emilio Fabian and Ana Paola Morales. On the other hand, Joselyn Gutiérrez, Maximiliano García and Marialinda Cadena were in charge of the development of the microfluidic simulations and designs as well as the liposome encapsulation nebulizer.

Wet Lab

Emilio Fabian was the main coordinator of the wet lab team, which performed all the necessary experimentation at the laboratory in order to confirm the functionality of the sRNAs and characterize them, along with the debugging of the different situations presented in the project. Ana Paola Morales was mainly in charge of the digestion protocols and PCR procedures, while Sofia Goitia and Juan Antonio were our main microbiologists ,electrophoresis , and purification protocol managers.

Software & Modeling

Juan Antonio Alfaro was the main coordinator of the Software & Modeling team, whom were responsible for the creation of a python-based pipeline for synthetic sRNA designing and an Artificial Intelligence model for predicting the behavior role (upregulation or downregulation) of the designed sRNAs.

Manuel, Hugo, Samyr and Juan Antonio performed bibliographical search in order to looking for current scoring methods and selection criteria for the designing of sRNAs and antisense RNAs in bacteria, while Juan Antonio designed the pipeline of the scoring method and Manuel, Hugo and Samyr selected and tested the required bioinformatic packages. Hugo Campos helped on the cleaning up of the code making it more understandable.

On the other hand, Manuel de Labra was the main responsible for the architecture design of the artificial intelligence in order to create a model for predicting sRNA regulation behavior. For training the artificial intelligence, Juan Antonio and Manuel Hernández looked for sRNA databases on bibliographical sources, while Juan Antonio designed the guidelines for filling-up the databases. Alejandra Velazquez, Ana Paula Rodriguez, Andrea Benitez, Andrea Gonzalez and Juan Manuel Dominguez plus all the previously mentioned members helped retrieving bibliography data for filling up the databases.

We want to thank Andres Alanis Quiroz, Carolina Nicole González Leal, Mi Dam Kim, Alejandra Lazo Barroeta and Edgar Oziel Hernández López, who helped us as collaborators by filling the reported databases.

Human Practices

Frida Nezhime Gallegos coordinated the Human Practices activities with the community. She was in charge of organizing the team's area weekly meetings and calendars. She contributed to the initial mapping of the problem. Sofia Elena Goitia and Emilio Fabian were the main contributors to the research and creation of the article: “Inequality in the Mexican Health System and its Repercussions in Antibiotic Resistance”. Sofia Elena Goitia was also in charge of creating and imparting antibiotic resistance awareness workshops, creating the antibiotic resistance survey, mapping the system, particiating in community activities, and contacting advisors. Emilio Fabian contributed to the mapping of the system. Ana Sofia Garza contributed to the article, participated in community activities and was in charge of submitting the information to the wiki.

Educational & Public Engagement

Angela Castillo and Alejandra Velazquez were in charge of the Educational projects. They supervised the research, writing and illustration, and edited the children 's book. They also designed and taught the biology classes to primary grade children. Minji Gallegos, Anya Gutierrez, Ana Laura Camacho and Alejandra Lazo created the illustrations for the book. Minji Gallegos and Francisco Romo wrote some of the scientists’ biographies. Alejandra Velázquez was in charge of the agar art and lung sculpture projects. Emilio Fabian provided help with the technical aspects of the educational and bioart projects.

Marketing

Ana Paula Rodríguez was the head of marketing and communications. She designed this year’s theme with the careful selection of colors and fonts, as well as for all the other projects iGEM Tec-Monterrey organized. Additionally, she was in charge of designing and scheduling the posts and stories for the team’s Instagram page. Ana Paula was also responsible for communicating with other iGEM teams, as well as setting up meetings for possible collaborations. Juan Vallejo, Mónica Rivas, and Marianne Castellanos contributed to the creation of posts and stories. Finally, Ana Paula also designed the layout of the wiki’s homepage and the general theme for the wiki.

Finance & Entrepreneurship

Adriana Salazar was the coordinator of Finance and she contributed by making invoices, updating the budget, the team´s pitches and creating sponsorships. She was in charge of the entrepreneurship section of the wiki. Andrea Benitez was the coordinator of Commercial Management. She contributed by preparing the material for contests, and by seeking sponsorships. She helped with the information of the entrepreneurship section of the wiki. Andrea González contributed with the contact and closing of sponsorships and helped with the information of the entrepreneurship section of the wiki. Marialinda and Regina were in charge of contributing with the information of the wiki. Adriana, Andrea Benítez and Andrea González contributed equally to the design of fundraising campaigns.

Strategic Planning and Projects

Andrea Chávez was the main leader of the area. During this year, she was in charge of carrying out the planning and structure of events that involved the participation of Human Practices and Educational, such as Biologix Summer Camp, Wednesday of Human, Human Day, and Synbio, as well as the administration of human capital, the building strategic relationships with physicians, researchers, and careers for purposes of iGEM. Yareli Xcarlett Amaya, supervised and coordinated the online sessions of Synbio, as well as the Wednesdays of human; Additionally, she directed the interviews with Dr. Marco Arturo Montell García and BPCs María Isaías Pineda Villanueva and Mónica Padua Ahumada. Juan Antonio Alfaro Almaguer and Ana Valeria García Lartigue planned the content of the summer camp, in the same way, they had active participation as teachers and staff members. Ana Valeria García was in charge of the planning and direct contact with researchers and leaders of other careers, taking care of public relations and the continuous improvement of the planning of events.

It is worth noting the active participation of Juan Antonio Alfaro Almaguer within this area since despite leading his mathematical model management at the same time, he was involved in the planning and implementation of each event, serving as teacher, coordinator, and moderator in each case.

Wiki

Ana Sofia Garza was the coordinator of wiki and contributed by organizing the all the project information, the photoshoots made for wiki and other usages, and help teaching other members of wiki to code. Gibrán, Carolina and Fausto contributed with the coding for the design of landing page and template for the wiki, to solve any error or doubt that could emerge. Ana Paula Rodríguez and Alejandra Velázquez contributed with the design for the landing page.

Instructors and advisors

Jesús Hernández (Chucho) was our main technical advisor, being with us since the beginning of our project, helping us with the debugging and evaluation of our results, providing us with reagents, and kidnapping our wet lab leader when needed. We want to make a special thank to Chucho for his time, knowledge, help and all the PCR plates and ladder that we stole from him (sometimes).
César Puente fulfilled the role of our Principal Instructor. He guided us through the justification of our project and helped us to establish connections as well as seeing the big picture whenever we could not. We want to thank him for his support and helping us with our shipment problems that significantly affected our project this year.
Silvia Hinojosa gave us bioinformatic technical advice and guidance towards the development of the conceptualization of our software, as well as keeping us company and good talk when doing minipreps in her laboratory. We want to thank her for all her good vibes and help provided.
Alejandro González provided us with an aliquot of a culture containing Pcomb 3x plasmid, which would be used to clone the part of the P3 protein that would give the M13 the ability to infect P. aeruginosa. We are very grateful for the support.
Erika Leal is the main coordinator of the laboratories at our institution. She facilitated us with laboratory access and equipment for the experimental part of our project.
Vianney Lara supported our student group and helped us obtain the necessary resources to participate in the iGEM Competition, in this sense we want to make a special thanks to “Fondo FEMSA” which allowed us to pay the entry fee required.
Raúl Villarreal Lara explained to us each of the components of the business model canvas, gave us advice, and helped us to focus our project on entrepreneurship.

Collaborators

Software & Model: Andres Alanis Quiroz, Carolina Nicole González Leal, Mi Dam Kim, Alejandra Lazo Barroeta, Edgar Oziel Hernández López.

Illustrations: Ana Paola Morales Mendoza.

Educational: Minji Gallegos, Anya Gutierrez, Ana Laura Camacho, Alejandra Lazo, Francisco Romo.

Finances & Entrepreneurship: Marialinda del Carmen Cadena Hernández, Regina Garza.

Marketing: Juan Salvador Vallejo Delgado, Mónica Rivas Elizondo, Marianne Michelle Castellanos Hobart.

Dry Lab: Regina Garza Garza, Luis Fernando Carmona Ramírez.

Human Practices: Alexa Paulin Liquidano, Anairis Navarro Montero, Mauricio Lopez.

Strategic Planning & Projects: Carlos Alonso Moreno Alcantar, Beatriz Nicolás Ruiz, Leslie Elizabeth Herrera Guerrero, Georgina Guadalupe Sarmiento Moncada.