This year, our team's experiments are mainly around three chassis: Escherichia coli, Synechococcus elongatus, and Azotobacter caulinodans. All of them are BSL-1 microorganisms, so our lab is qualified enough to engineer them.

Every year, our school conducts a laboratory safety knowledge test to urge everyone to learn laboratory safety. Only those who pass the exam are qualified to enter the laboratory. Before the start of this year's experiment, all team members will have laboratory safety training and sign a safety commitment. In the process of the experiment, we strictly follow the university of Science and Technology laboratory safety inspection management method. Our laboratory guidelines include but are not limited to:

1. The instruction of the safety information board must be clear, with a safety inspection and health duty management system.
2. The ventilation system of the experimental site is in normal operation, the fume hood and super clean bench can be used normally, and the cabinet door is opened at an appropriate height. Put the goods neatly, and do not pile up debris.
3. When entering the laboratory, you should wear personal protective equipment as required. You should not leave the laboratory wearing PPE such as contaminated lab clothes and gloves.
4. Food and drink must not be present in the laboratory.
5. The laboratory has an emergency evacuation route map, and the fire escape is unobstructed. The laboratory is equipped with suitable fire-fighting equipment, emergency spray, eyewash, and first aid kit, which are regularly maintained.
6. Any dangerous use of electricity is prohibited in the laboratory.
7. The hazardous chemicals comply with the relevant regulations of the state and the school. Laboratory personnel should understand the hazardous characteristics of the chemicals used, safety protection knowledge, storage methods, waste treatment, emergency treatment methods, etc.
8. Hazardous waste and general waste shall be stored separately, and hazardous waste labels shall be posted.
9. Waste fluids containing bacteria need to be sanitized before being poured down the drain.

Our Mini Bioproduction Cycle System is planned to be used on the Mars base. Therefore, our project design needs to suit for the application of extraterrestrial planets.

First, the chassis we used were all BSL-1 microorganisms. As far as we know, BSL-1/2 organisms are allowed to be taken on the space station.^[1] So we believe that BSL1 microorganisms should also be available for the Mars bases.

Second, the design of immobilized fermentation improves the biosafety of our projects. Immobilization reduces the possibility of bacteria leaking into the environment. Also, the immobilized bacteria can be transported more safely in space, while the liquid cultures may leak in transit due to weightlessness.

[1] Wong, Wing & Guidry, Richard & Arneson, David & Zimmerman, Domenica & Downing, Marian & Castro, Victoria & Pierson, Duane. (2011). Biosafety Onboard the International Space Station. Applied Biosafety. 16. 158-162. 10.1177/153567601101600304.