Protocols

For cloning process of all our modules (except Dextran), we followed standard iGEM 3A Assembly and Transformation. The rest of our module specific protocols are as follows:





Asparagine Module

Synthesis and Validation of Asn-BSA Beads


Download Synthesis and Validation of Asn-BSA Beads Protocol

EibA/D Experiments


Download EibA/D Experiment Protocol

Eib Induction and Agglutination Assay


Download Eib Induction and Agglutination Assay Protocol

Choline Module

Protein Induction and Measuring Expression


Download Protein Induction and Measuring Expression Protocol

Large Scale Protein Purification and Buffer Exchange


Download Large Scale Protein Purification and Buffer Exchange Protocol

ChOx Enzyme Activity Testing


Download ChOx Enzyme Activity Testing Protocol

Sarcosine Module

Method 1: Gold Nanoparticles


Download Method 1: Gold Nanoparticles Protocol

Method 2: Chemical rGO + Chitosan


Download Method 2: Chemical rGO + Chitosan Protocol

Method 3-5


Download Method 3-5 Protocol

Dextran Module

Isolation of Dextran


Download Isolation of Dextran Protocol

Dextran Hydrogels via N,N0 - methylenebisacrylamide crosslinking


Download Dextran Hydrogels via N,N0 - methylenebisacrylamide crosslinking Protocol

Dextran Hydrogel Characterization


Download Dextran Hydrogel Characterization Protocol