Experiments

experiment1

General Experimental Protocols


notebooks

Preparing Overnight Cultures

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Miniprep Protocol (Qiagen)

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Making New LB Media

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LB+Agar Media and Agar Plates

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Cell Fixation

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qPCR

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Making a Replication of Plates

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Making an Agarose Gel

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Making TAE Buffer

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Restriction Enzyme Digest

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E. Coli Electroporation

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Gel Extraction

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Heat Shock Transformation

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Creating Electrocompetent Cells

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Making Oligo Stock

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Diluting Oligo Stock 1:10

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Overnight Cells in Glycerol

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Gibson Assembly

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