Our team planned to use 12 basic parts. We inserted the B5R gene (with 1st sushi and all 4 sushi ectodomains) into pET23a plasmid transformed into E. coli BL21(DE3) (no. 1 and 2). We used 3 primers for B5R gene cloning from pVAX plasmid (one forward - no. 8 - and two reverse - no. 9 and 10). Other possible genetic models for B5R protein synthesis can be Codon optimized B5R (all 4 sushi domains) sequence (no. 11) and B5R sequence (all 4 sushi domains) with altered Cys140 to Ser (no. 12).
Moreover, since we used the L1, A33, and A27 proteins of the Vaccinia virus for optic fiber functionalization, we included them as other basic parts (no. 5-7). We also included aptamer sequences for L1 protein as the example reference for aptamer modeling in MAWS software made by the iGEM Heidelberg 2021 team.