Attributions

    The iGEM team of NJXDF-CHN is responsible for almost all aspects of team operation. This year, our team was divided into four groups: artists, Wiki, experimental and HP, which work effectively. Our idea was proposed by brainstorming combined with realistic questions, and idea was improved through human practice activities. All the work done by us has been shown below.

    The captain of the overall planning and experimental group; participated in the production of promotional video; participated in the planning of education, publicity and other activities.

    The leader of the dry team; responsible for the planning of education and publicity activities; participated in the production of promotional video.

    The leader of the experimental group; responsible for the distribution of experimental tasks and the progress of the experiment; screened the optimal phenylacetaldehyde reductase by using the NCBI Blasting function.

    Responsible for the production of publicity picture books; participated in the construction and design of Wiki; participated in the production of promotional video.

    Member of the dry team; participated in the construction and design of Wiki; participated in the production of team uniforms and team logo.

    Member of the dry team; participated in the construction and design of Wiki; participated in some expert exchange activities.

    Member of the dry team; participated in the production of the presentation documents; participated in the production and collection of questionnaires.

    Member of the dry team; participated in the production of the presentation documents; participated in the production and collection of questionnaires.

    Member of the dry team; participated in the production of promotional video.

    Member of the dry team; participated in the production of promotional video.

    Member of the dry team; participated in the planning of education, publicity and other activities; participated in the production of team uniforms and team logo.

    Member of the dry team; participated in the planning of education, publicity and other activities.

    Member of the dry team; participated in material count and preparation;participated in the production of promotional video.

    Member of the dry team; participated in the production and collection of questionnaires; participated in the production of team uniforms and team logo.

    Member of the experimental group; constructed plasmid pYLXP-ADH1, pYLXP-ADH2, pYLXP-ADH3, and pYLXP-ADH4; participated in the production of the presentation documents.

    Member of the experimental group; constructing plasmid pYLXP-ARO8 and pYLXP-ARO10; participated in some expert exchange activities.

    Member of the experimental group; constructing plasmid pYLXP’-ADH5, pYLXP’-ADH6, pYLXP’-ADH7, pYLXP’-ADH8, and pYLXP’-ADH9.

    Member of the experimental group; constructing plasmid pYLXP-ARO10-ARO8 and pYLXP-ARO10-ARO9.

    Member of the experimental group; constructing plasmid pYLXP-PAR1, pYLXP-PAR2, pYLXP-PAR3 and pYLXP-PAR4; participated in some expert exchange activities.

    Member of the experimental group; constructing plasmid pYLXP-ARO10-ARO8-PAR4; participated in the Contribution of BBa_ K3753000 part characterization.

    Member of the experimental group; constructing plasmid pYLXP-PAR5, pYLXP-PAR6， pYLXP-PAR7, and pYLXP-PAR8.

    Member of the experimental group; constructing plasmid pYLXP-PARL; performing the shaking flask of engineered strain po1fk3.

    Member of the experimental group; performing yeast transformation and optimized screening of high-producing strains.

    Member of the experimental group; participated in the contribution of BBa_ K2533034 part characterization; participated in making and collection of questionnaires.

    Member of the experimental group; performing the whole-cell biocatalyst; participated in the contribution of BBa_ K4180002 part characterization.

    Member of the experimental group; performing the shaking flask of engineered strain po1g1, po1g2, and po1g3; participated in the production of promotional video.

    Member of the experimental group; performing the quantification of cell density, 2-PE, penylacetate, L-phenylalanine.

    Thanks to our Primary PI Dr. Yang Gu for organizing NJXDF-CHN and providing materials and instruments.

    We thank our secondary PIs and instructors Mrs. Hongyan Wang, Dr. Xiaoman Sun, Dr. Chao Ye, and Dr. Qingzhuo Wang, for their inspiration and guidance from all aspects, put forward general suggestions, and help optimize experiments, manage our laboratory space, and teach us to develop the technology of biology.

    Thanks to Dr. Hao Wang for giving us a fundamental knowledge of the current use of 2-PE.

    Thanks to Jiaqi Xu for proposing methods to optimize expression using the microbial system in order to address the problem of improving low 2-PE production.

    Thanks to Dr. Zhengxiong Zhou for directing our molecular biology experiment.

    Thanks to Nanjing Customs for providing us with information about 2-PE in our daily life.

    Thanks to NNU-China, Jiangnan-China, NPU-China, Jilin_China, Guangxi-U-China for working with our team.

    Thanks to the School of Food and Pharmaceutical Engineering of Nanjing Normal University for our strong support.

    Last but not least, thanks to all members in NJXDF-CHN!