NJU-China 2022 iGEM Team Parts

We have used or made several parts during the whole process of our project, each of which has its own functions and features. This year's new parts were integrated with the wisdom of our team members Haoyu Sun and they all displayed different results we expected. Here are the details of our parts, you can click the links for more information.

Basic Parts

Part Number	Part Name	Part Type	Abstract	Matching Medal
BBa_K4173021	β-catenin	Coding	The gene not only encodes classical calcium mucin adhesion complex which is not only an important composition of the cytoplasm that formed in the epithelial cells of the adhesive connection but also mediates cell-cell adhesion of many other organizations, encodes Wnt signaling pathways in the typical key signaling molecule which is the signaling pathways in normal development to control in the process of tumor cell growth and differentiation. The gene product contains a central armadillo repeat domain through which it binds to the cytoplasmic tail of classical cadherins. At the same time, it also binds α-catenin, further linking the cadherin complex directly or indirectly to the actin cytoskeleton. So β-catenin is necessary for the adhesion of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. If there is an absence of of Wnt signaling, cytoplasm-serial protein which has nothing to do with the calcium mucin complexes serine/threonine residues on the n end on the degradation of so-called complex is fast phosphorylated. The degradation of protein complexes containing axis, adenomatous escherichia coli (APC), casein kinase I and GSK3B might play, and this complexes then go through TRCP ubiquitin and proteasome degradation. However, in the presence of Wnt signaling, the degradation complex is disrupted and stable cytoplasmic -catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations in this gene have been linked to various types of tumors. Splice variants of the gene have also been found.	Silver
BBa_K4173022	CD63	Coding	CD63 is a member of the tetraspanin superfamily of activation-linked cell surface antigens with N-terminal and C-terminal intracellular domains, two link domains and two annular extracellular domains, and is one of the most widely used exosome markers(CD63, CD9, CD81). More recently, however, their presence in other EVs has been observed. According to the latest study, CD63 was proved to be the specific marker for exosome, while EVs bearing only CD9 or CD81 but not CD63 probably did not form in endosomes. CD63 is known for its abnormally high levels on the surface of activated basophils, on proliferating mast cells, and on the surface of endothelial cells in inflamed tissue. CD63 plays an important role in the regulation of cargo sorting and vesicle formation. As a transmembrane protein located specifically on exosomes, it can be used as a base protein for fusing other proteins on which help the target protein locate on the membrane and be packaged into exosomes.	Silver
BBa_K4173024	L7Ae	Coding	L7Ae is an archaeal ribosomal protein 7Ae 60S large ribosomal subunit, a subunit of archaeal RNase P, plays multiple roles in archaea. It is part of the H/ACA and the C/D box snoRNPs, which catalyze rRNA pseudouridylation and 2’-O-methylation respectively. Thus, L7Ae is one of the most commonly used riboswitches which RNA-only delivery gene circuits highly rely on except RNA binding proteins (RBP). L7Ae, a K-turn and K-loop binding protein, is a component of box C/D RNPs and interacts with the K-turn motifs of archaeal box C/D sRNAs3. Through the interaction between L7Ae and C/D box RNA structure which is added at UTR of reporter gene, the target protein can be grasped and stay close to L7Ae. Then target protein can be held on the membrane of exosomes when L7Ae is conjugated to the C-terminus of CD63. Finally, L7Ae-CD63 can serve as an active packaging device of specific RNAs into exosomes.	Silver
BBa_K4173045	pcDNA3.1-box CD mini- β catenin-mCherry	Plasmid	This DNA circuit is designed to express specific protein. It can express beta catenin.The gene not only encodes classical calcium mucin adhesion complex which is not only an important composition of the cytoplasm that formed in the epithelial cells of the adhesive connection but also mediates cell-cell adhesion of many other organizations, encodes Wnt signaling pathways in the typical key signaling molecule which is the signaling pathways in normal development to control in the process of tumor cell growth and differentiation. The gene product contains a central armadillo repeat domain through which it binds to the cytoplasmic tail of classical cadherins. At the same time, it also binds α-catenin, further linking the cadherin complex directly or indirectly to the actin cytoskeleton. So β-catenin is necessary for the adhesion of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. If there is an absence of of Wnt signaling, cytoplasm-serial protein which has nothing to do with the calcium mucin complexes serine/threonine residues on the n end on the degradation of so-called complex is fast phosphorylated. The degradation of protein complexes containing axis, adenomatous escherichia coli (APC), casein kinase I and GSK3B might play, and this complexes then go through TRCP ubiquitin and proteasome degradation. However, in the presence of Wnt signaling, the degradation complex is disrupted and stable cytoplasmic -catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations in this gene have been linked to various types of tumors. Splice variants of the gene have also been found.	Silver
BBa_K4173046	pcDNA3.1-CD63-L7Ae-mCherry	Plasmid	This DNA circuit is designed to express specific proteins. It can express the fusion protein CD63-L7Ae. CD63 is a member of the tetraspanin superfamily of activation-linked cell surface antigens with N-terminal and C-terminal intracellular domains, two link domains and two annular extracellular domains, and is one of the most widely used exosome markers(CD63, CD9, CD81). More recently, however, their presence in other EVs has been observed. According to the latest study, CD63 was proved to be the specific marker for exosome, while EVs bearing only CD9 or CD81 but not CD63 probably did not form in endosomes. CD63 is known for its abnormally high levels on the surface of activated basophils, on proliferating mast cells, and on the surface of endothelial cells in inflamed tissue. CD63 plays an important role in the regulation of cargo sorting and vesicle formation. As a transmembrane protein located specifically on exosomes, it can be used as a base protein for fusing other proteins on which help the target protein locate on the membrane and be packaged into exosomes. L7Ae is an archaeal ribosomal protein 7Ae 60S large ribosomal subunit, a subunit of archaeal RNase P, plays multiple roles in archaea. It is part of the H/ACA and the C/D box snoRNPs, which catalyze rRNA pseudouridylation and 2’-O-methylation respectively. Thus, L7Ae is one of the most commonly used riboswitches which RNA-only delivery gene circuits highly rely on except RNA binding proteins (RBP). L7Ae, a K-turn and K-loop binding protein, is a component of box C/D RNPs and interacts with the K-turn motifs of archaeal box C/D sRNAs3. Through the interaction between L7Ae and C/D box RNA structure which is added at UTR of reporter gene, the target protein can be grasped and stay close to L7Ae. Then target protein can be held on the membrane of exosomes when L7Ae is conjugated to the C-terminus of CD63. Finally, L7Ae-CD63 can serve as an active packaging device of specific RNAs into exosomes.	Silver
BBa_K4173048	pcDNA3.1-si5alphaR1-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA. We also designed other five siRNAs with the same function, and this one proved to be the most effective.	Silver
BBa_K4173049	pcDNA3.1-si5alphaR2-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173050	pcDNA3.1-si5alphaR3-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173051	pcDNA3.1-si5alphaR4-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173052	pcDNA3.1-si5alphaR5-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173053	pcDNA3.1-si5alphaR6-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target 5alphaR and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173054	pcDNA3.1-siPiezo1-1-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173055	pcDNA3.1-siPiezo1-2-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173056	pcDNA3.1-siPiezo1-3-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173057	pcDNA3.1-siPiezo1-4-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA.	Bronze
BBa_K4173058	pcDNA3.1-siPiezo1-5-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA. We also designed other five siRNAs with the same function, and this one proved to be the most effective.	Silver
BBa_K4173059	pcDNA3.1-siPiezo1-6-mCherry	Plasmid	This DNA circuit is designed to express specific siRNA that specifically target Piezo1 and down-regulate the expression level of relevant target mRNA.	Bronze

Composite Parts

Part Number	Part Name	Part Type	Abstract	Matching Medal
BBa_K4173000	5αR-siRNA-1	RNA	This part expresses siRNA for 5αR, which can specifically degrade 5αR. 5αR is a reductase that catalyzes the conversion of testosterone to DHT. DHT causes the atrophy and closure of hair follicle cells through a series of downstream signaling pathways, which ultimately leads to androgen alopecia. The siRNA we used specifically binds to DHT reductase 5αR mRNA, thereby inhibiting the translation of this gene and downregulating the 5αR level in hair follicle cells, thereby reducing the effect of androgen (testosterone) on hair follicle cells and thereby inhibiting androgen alopecia. We also designed other five siRNAs with the same function, and this one proved to be the most effective.	Silver
BBa_K4173001	5αR-siRNA-2	RNA	This part expresses siRNA for 5alphaR, which can specifically degrade 5alphaR. We compare it with another five 5alphaR-siRNAs to select the best siRNA.	Bronze
BBa_K4173002	5αR-siRNA-3	RNA	This part expresses siRNA for 5alphaR, which can specifically degrade 5alphaR. We compare it with another five 5alphaR-siRNAs to select the best siRNA.	Bronze
BBa_K4173003	5αR-siRNA-4	RNA	This part expresses siRNA for 5alphaR, which can specifically degrade 5alphaR. We compare it with another five 5alphaR-siRNAs to select the best siRNA.	Bronze
BBa_K4173004	5αR-siRNA-5	RNA	This part expresses siRNA for 5alphaR, which can specifically degrade 5alphaR. We compare it with another five 5alphaR-siRNAs to select the best siRNA.	Bronze
BBa_K4173005	5αR-siRNA-6	RNA	This part expresses siRNA for 5alphaR, which can specifically degrade 5alphaR. We compare it with another five 5alphaR-siRNAs to select the best siRNA.	Bronze
BBa_K4173006	Piezo1-siRNA-1	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. We compare it with another five Piezo1-siRNAs to select the best siRNA.	Bronze
BBa_K4173007	Piezo1-siRNA-2	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. We compare it with another five Piezo1-siRNAs to select the best siRNA.	Bronze
BBa_K4173008	Piezo1-siRNA-3	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. We compare it with another five Piezo1-siRNAs to select the best siRNA.	Bronze
BBa_K4173041	Piezo1-siRNA-4	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. We compare it with another five Piezo1-siRNAs to select the best siRNA.	Bronze
BBa_K4173042	Piezo1-siRNA-5	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. Piezo1 is a mechanically gated cation channel in cell membranes. Piezo1 has been used for the treatment of weightlessness related diseases. According to literature review, Piezo1 is also abnormally activated in the process of apoptosis of hair follicle stem cells, and inhibiting the function of Piezo1 receptor can alleviate the apoptosis of hair follicle stem cells caused by mechanical stress. Meanwhile, up-regulation of apoptosis in hair follicle stem cells was also observed during androgen alopecia. Our siRNA can inhibit the translation of Piezo1 gene by binding to its mRNA, thereby down-regulating the level of Piezo1 receptor on cell membrane and inhibiting the apoptosis of hair follicle stem cells during androgen alopecia. We also designed other five siRNAs with the same function, and this one proved to be the most effective.	Silver
BBa_K4173043	Piezo1-siRNA-6	RNA	This part expresses siRNA for Piezo1, which can specifically degrade Piezo1. We compare it with another five Piezo1-siRNAs to select the best siRNA.	Bronze