Basic parts
Promoter
| name | part name | part type | function and description | length | designer |
|---|---|---|---|---|---|
| BBa_K4121008 | pCCW12 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Qi |
| BBa_K4121018 | pTDH1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Qi |
| BBa_K4121012 | pFBA1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Qi |
| BBa_K4121005 | pACT1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Lu Yiwei |
| BBa_K4121015 | pTPI1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Lu Yiwei |
| BBa_K4121016 | pTDH2 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Lu Yiwei |
| BBa_K4121011 | pPGK1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 781bp | Lu Yiwei |
| BBa_K4121006 | pYMP122W-A | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Wanyi |
| BBa_K4121013 | pRFS23A | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Wanyi |
| BBa_K4121017 | pPGL1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Hu Yiyi |
| BBa_K4121010 | pRPS27B | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Li Wanyi |
| BBa_K4121007 | pTEF1 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Wang Yanwei |
| BBa_K4121014 | pTDH3 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 501bp | Wang Yanwei |
| BBa_K4121009 | pXHT7 | constitutive promoter | Turn on transcription of downstream genes in Saccharomyces cerevisiae | 485bp | Wang Yanwei |
CDS
| name | part name | part type | function and description | length | designer |
|---|---|---|---|---|---|
| BBa_K4121019 | tHMG | protein coding sequences | The N-terminal interception gene tHMG1 of HMG-CoA reductase encoding gene HMG1 in mevalonate pathway.HMG1 has the function of catalyzing the conversion of HMG-CoA to mevalonate. | 1592bp | Song Chaonan |
| BBa_K4121025 | idi | protein coding sequences | Isopentenyl diphosphate isomerase (IDI) is a key rate-limiting enzyme in terpene biosynthesis, catalyzing the interconversion between IPP and DMAPP in mevalonate pathway. | 864bp | Zhang Qiuhui |
| BBa_K4121026 | crtE | protein coding sequences | Catalyze the conversion of FPP to GGPP | 1179bp | Song Chaonan |
| BBa_K4121028 | crtB | protein coding sequences | Catalyze the conversion of GGPP to phytoene | 888bp | Song Chaonan |
| BBa_K4121027 | crtI | protein coding sequences | Catalyze the conversion of phytoene to lycopene | 1746bp | Song Chaonan |
| BBa_K4121023 | ERG8 | protein coding sequences | Catalyze the conversion of mevalonate-P to mevalonate-PP in mevalonate pathway | 1352bp | Zhang Qiuhui |
| BBa_K4121020 | ERG10 | protein coding sequences | Catalyze the conversion of Acetyl-CoA to Acetoacetyl-CoA in mevalonate pathway | 1197bp | Zhang Qiuhui |
| BBa_K4121022 | ERG12 | protein coding sequences | Catalyze the conversion of mevalonate to mevalonate-P in mevalonate pathway | 1331bp | Zhang Qiuhui |
| BBa_K4121021 | ERG13 | protein coding sequences | Catalyze the conversion of Acetoacetyl-CoA to HMG-CoA in mevalonate pathway | 1473bp | Zhang Qiuhui |
| BBa_K4121024 | ERG19 | protein coding sequences | Catalyze the conversion of mevalonate-PP to IPP in mevalonate pathway | 1188bp | Zhang Qiuhui |
Selection marker
| name | part name | part type | function and description | length | designer |
|---|---|---|---|---|---|
| BBa_K4121029 | his | amino acid coding sequence | As a screening marker for four-deficient Saccharomyces cerevisiae | 660bp | Song Chaonan |
| BBa_K4121032 | trp | amino acid coding sequence | As a screening marker for four-deficient Saccharomyces cerevisiae | 675bp | Song Chaonan |
| BBa_K4121030 | leu | amino acid coding sequence | As a screening marker for four-deficient Saccharomyces cerevisiae | 1095bp | Song Chaonan |
| BBa_K4121031 | ura | amino acid coding sequence | As a screening marker for four-deficient Saccharomyces cerevisiae | 1095bp | Song Chaonan |
Homologous arm
| name | part name | part type | function and description | length | designer |
|---|---|---|---|---|---|
| BBa_K4121033 | URR1 | homologous arm sequences | Single-copy homologous arms on the Saccharomyces cerevisiae genome | 535bp | Zheng Liming |
| BBa_K4121034 | URR2 | homologous arm sequences | Single-copy homologous arms on the Saccharomyces cerevisiae genome | 535bp | Zheng Liming |
| BBa_K4121035 | Ty1 | transposon sequences | Transposon sequences on the Saccharomyces cerevisiae genome | 447bp | Zheng Liming |
| BBa_K4121036 | Ty2 | transposon sequences | Transposon sequences on the Saccharomyces cerevisiae genome | 502bp | Zheng Liming |
Terminator
| name | part name | part type | function and description | length | designer |
|---|---|---|---|---|---|
| BBa_K4121038 | tENO1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121039 | tSSA1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121040 | tADH1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121041 | tPGK1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121042 | tENO2 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121043 | tTDH1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121044 | tADH2 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121045 | tAOX1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121046 | tCYC1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 225bp | Zhang Ruilin |
| BBa_K4121047 | tCPS1 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 485bp | |
| BBa_K4121048 | tPRM9 | terminator | Terminate transcription of upstream genes in Saccharomyces cerevisiae | 250bp |
composite parts
| name | part name | part type | function and description | designer |
|---|---|---|---|---|
| BBa_K4121051 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Hu Yiyi |
| BBa_K4121052 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Hu Yiyi |
| BBa_K4121053 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Hu Yiyi |
| BBa_K4121054 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Hu Yiyi |
| BBa_K4121055 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Hu Yiyi |
| BBa_K4121056 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121057 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121058 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121059 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121060 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121061 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121062 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121063 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121071 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121072 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121073 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of FPP to GGPP | Zhang Qiuhui |
| BBa_K4121074 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121075 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121076 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Hu Yiyi |
| BBa_K4121077 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Hu Yiyi |
| BBa_K4121078 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Hu Yiyi |
| BBa_K4121079 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121080 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121068 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121069 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121066 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of phytoene to lycopene | Zhang Qiuhui |
| BBa_K4121067 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of GGPP to phytoene | Zhang Qiuhui |
| BBa_K4121064 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of GGPP to phytoene | Zhang Qiuhui |
| BBa_K4121065 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of GGPP to phytoene | Zhang Qiuhui |
| BBa_K4121070 | The expression cassette of TmCrtE with a constitutive promoter | composite part | Catalyze the conversion of GGPP to phytoene | Zhang Qiuhui |
| BBa_K4121081 | The expression cassette of ERG8 with a constitutive promoter | composite part | Catalyze the conversion of mevalonate-P to mevalonate-PP in mevalonate pathway | Zhang Qiuhui |
| BBa_K4121082 | The expression cassette of ERG10 with a constitutive promoter | composite part | Catalyze the conversion of Acetyl-CoA to Acetoacetyl-CoA in mevalonate pathway | Zhang Qiuhui |
| BBa_K4121083 | The expression cassette of ERG12 with a constitutive promoter | composite part | Catalyze the conversion of mevalonate to mevalonate-P in mevalonate pathway | Zhang Qiuhui |
| BBa_K4121084 | The expression cassette of ERG13 with a constitutive promoter | composite part | Catalyze the conversion of Acetoacetyl-CoA to HMG-CoA in mevalonate pathway | Zhang Qiuhui |
| BBa_K4121085 | The expression cassette of ERG19 with a constitutive promoter | composite part | Catalyze the conversion of mevalonate-PP to IPP in mevalonate pathway | Zhang Qiuhui |
| BBa_K4121086 | The expression cassette of IDI with a constitutive promoter | composite part | Isopentenyl diphosphate isomerase (IDI) is a key rate-limiting enzyme in terpene biosynthesis, catalyzing the interconversion between IPP and DMAPP in mevalonate pathway. | Zhang Qiuhui |
