Parts


We created the following parts as part of our project this year. Basic parts include our AMP (HBCM2), the T4GALA encapsulin we used, and the TEV + targeting peptide sequence we used to facilitate AMP encapsulation. Our composite part is our experimental construct that consists of the AMP + targeting peptide + encapsulin. Our AMP and targeting peptide were synthesized by IDT. Our encapsulin was generously donated to us by the Giessen lab as part of a plasmid and isolated using PCR. Our composite part was assembled using Gibson assembly.

The following table describes the parts we used over the course of our project this year.


Name Type Description Length (bp)
BBa_K4143336 Basic Antimicrobial Peptide: HBCM2 78
BBa_K4143337 Basic T4GALA Encapsulin 994
BBa_K4143339 Basic TEV Protease + T4GALA Encapsulin Targeting Peptide 90
BBa_K4143340 Composite AMP Encapsulin Composite 1119