We created the following parts as part of our project this year. Basic parts include our AMP (HBCM2), the T4GALA encapsulin we used, and the TEV + targeting peptide sequence we used to facilitate AMP encapsulation. Our composite part is our experimental construct that consists of the AMP + targeting peptide + encapsulin. Our AMP and targeting peptide were synthesized by IDT. Our encapsulin was generously donated to us by the Giessen lab as part of a plasmid and isolated using PCR. Our composite part was assembled using Gibson assembly.
The following table describes the parts we used over the course of our project this year.
| Name | Type | Description | Length (bp) |
|---|---|---|---|
| BBa_K4143336 | Basic | Antimicrobial Peptide: HBCM2 | 78 |
| BBa_K4143337 | Basic | T4GALA Encapsulin | 994 |
| BBa_K4143339 | Basic | TEV Protease + T4GALA Encapsulin Targeting Peptide | 90 |
| BBa_K4143340 | Composite | AMP Encapsulin Composite | 1119 |