Parts created by KEYSTONE in this season
| Utilized Existing Parts | |||
|---|---|---|---|
| Part name | Part number | Contribution | Link |
| ptac-RiboJ | BBa_K3552015 | As an IPTG-inducible promoter, we used it to express our santalene-producing enzymes including santalene synthase and ERG20. | BBa_K3552015 |
| B0034 | BBa_B0034 | As a biobrick RBS (Ribosome-Binding Site), we used it to be assembled with several CDS, such as ClSS_S533A (Part Number: BBa_K4274000) ,CmR29M2-ClSS_S533A (Part Number:BBa_K4274001),ERG20 (Part Number: BBa_K849001), ERG20_F96W(Part Number: BBa_K4274002). | BBa_B0034 |
| B0015 | BBa_B0015 | This is one of the most commonly used terminators and is also one of the most reliable ones. This was also used with several CDS such as ClSS_S533A (Part Number: BBa_K4274000) and ERG20_F96W (Part Number: BBa_K4274002). | BBa_B0015 |
| ERG20 | BBa_K849001 | We engineered E.coli Strain DH5a（tnaA-） to heterologously express ERG20 to test the yield of santalene. | BBa_K849001 |
| p43 | BBa_K143013 | The gene coded for the over-expression of degQ which has been knocked into the B. subtilis. | BBa_K143013 |
| New Parts | |||||
|---|---|---|---|---|---|
| Part name | Part number | Sequence correct? | Does it function? | Group favorite | Link |
| ClSS_S533A | BBa_K4274000 | Yes | Yes | Yes | BBa_K4274000 |
| CmR29M2-ClSS_S533A | BBa_K4274001 | Yes | Yes | N/A | BBa_K4274001 |
| ERG20_F96W | BBa_K4274002 | Yes | Yes | Yes | BBa_K4274002 |
| ClSS_S533A-FL-ERG20_F96W | BBa_K4274003 | Yes | Yes | N/A | BBa_K4274003 |
| PmanP | BBa_K4274005 | Yes | Yes | Yes | BBa_K4274005 |
| PvanP* | BBa_K4274006 | Yes | Yes | Yes | BBa_K4274006 |
| PatB_target(gRNA) | BBa_K4274007 | Yes | Yes | Yes | BBa_K4274007 |
| Sfp_target(gRNA) | BBa_K4274008 | Yes | Yes | Yes | BBa_K4274008 |
| sfp | BBa_K4274009 | Yes | Yes | Yes | BBa_K4274009 |
| degQ | BBa_K4274010 | Yes | Yes | Yes | BBa_K4274010 |
| sfp_HA_US | BBa_K4274011 | Yes | Yes | Yes | BBa_K4274011 |
| sfp_HA_DS | BBa_K4274012 | Yes | Yes | Yes | BBa_K4274012 |
| Synthetic RBS1 in pJOE8999 | BBa_K4274013 | Yes | Yes | Yes | BBa_K4274013 |
| Synthetic RBS2 in pJOE8999 | BBa_K4274014 | Yes | Yes | Yes | BBa_K4274014 |
| Composite Parts | ||||||
|---|---|---|---|---|---|---|
| Part name | Part number | Composite of | Sequence correct? | Does it function? | Group favorite | Link |
| ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015 | BBa_K4274030 | BBa_K3552015, BBa_B0034, BBa_K4274000, BBa_B0034,BBa_K849001,BBa_B0015 | Yes | Yes | N/A | BBa_K4274030 |
| ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20_F96W-B0015 | BBa_K4274031 | BBa_K3552015, BBa_B0034, BBa_K4274000, BBa_B0034,BBa_K4274002,BBa_B0015 | Yes | Yes | N/A | BBa_K4274031 |
| ptac-RiboJ-B0034-CmR29M2_ClSS_S533A-B0034-ERG20_F96W-B0015 | BBa_K4274032 | BBa_K3552015, BBa_B0034, BBa_K4274001, BBa_B0034,BBa_K4274002,BBa_B0015 | Yes | Yes | N/A | BBa_K4274032 |
| ptac-RiboJ-B0034-ClSS_S533A-FL-ERG20_F96W-B0015 | BBa_K4274033 | BBa_K3552015, BBa_B0034, BBa_K4274003,BBa_B0015 | Yes | Yes | N/A | BBa_K4274033 |
| PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS | BBa_K4274035 | BBa_K4274006,BBa_K4274008,BBa_K4274011,BBa_K143013,BBa_K4274013,BBa_K4274009,BBa_K4274014,BBa_K4274010,BBa_K4274012 | Yes | Yes | N/A | BBa_K4274005 |
| PvanP*-patB_target | BBa_K4274036 | BBa_K4274006,BBa_K4274007 | Yes | Yes | N/A | BBa_K4274036 |