Team Attributions

The KCIS iGem team was fortunate enough to be provided with an opportunity
to compete alongside many other innovative teams.
The experiences and lessons we gained from this opportunity were truly unforgettable,
so our team is thankful from the bottom of our hearts.
We are also grateful for all the advice and support given to us by a variety of different groups throughout this journey,
and we will do our best to acknowledge their contributions on this webpage,
as well as give an overview of the responsibilities of our team members





Introduction and Training

Classifiction Name
Content Review/ Editing Tarn Parker, Hui Ching Kuo
Laboratory Training/Supervision Hui Ching Kuo

Team Leaders

Classifiction Name
Worked with faculty and advisors from the very start of the project to obtain all the support for our team. Involved in all aspects of the project: from creating assignments for each group to overseeing each group’s progress, and making sure our project was running smoothly and produced the best possible quality. Charlotte Hsu and Terry Cheng

Project Brainstorming and Formation

Classifiction Name
Topic Selection Phoebe Lin, Grace Chang, Kai Hsiao, Felix Tsai, Terry Cheng, Laurence Chen, Andrew Chou
Literature Review Whole team
Wiki Write-Up Whole team

Experimental

Classifiction Name
Literature Review + Planning Whole team
Gel Electrophoresis Whole team
Competent Cell and Plasmid Transformation Whole team
PCR Whole team
Bacterial Plasmid Extraction Whole team
T4 Ligation Whole team
Yeast Genomic DNA extraction Whole team
Yeast RNA extraction Whole team
RT-qPCR Whole team
Phenol Chloroform extraction Whole team
NEB Enzyme Digestion Whole team
Wiki Write-Up Victoria Wang, Hao-Hung Chen, Phoebe Lin
Notebook Felix Tsai, Hao-Hung Chen, Victoria Wang, Lira Lee, Kai Hsiao, Laurence Chen

Human Practices

Classifiction Name
ASIJ collaboration Lira Lee, Hao-Hung Chen, Charlotte Hsu, Andrew Chou, Terry Cheng
NYCU Formosa collaboration Charlotte Hsu, Andrew Chou, Terry Cheng, Laurence Chen
Taiwan August Synbio Alliance Conference Victoria Wang, Phoebe Lin, Terry Cheng, Charlotte Hsu
Wego High school collaboration Lira Lee, Hao-Hung Chen, Charlotte Hsu, Andrew Chou, Terry Cheng
TEC Chihuahua collaboration Kate Wang, Andrew Chou
Korean HS collaboration Charlotte Hsu, Andrew Chou, Terry Cheng
iGEM newspaper Grace Chang, Victoria Wang, Lira Lee, Hao-Hung Chen
Social media team Grace Chang, Charlotte Hsu, Lira Lee, Phoebe Lin, Kai Hsiao, Luigi Wang
Interviews Andrew Chou
Wiki Content Andrew Chou, Grace Chang
Feedback analysis and synopsis Grace Chang

Lesson Outreach

Classifiction Name
Promotional Video Grace Chang, Andrew Chou, Terry Cheng, Victoria Wang, Felix Tsai, Kai Hsiao, Hao-Hung Chen
Education seminar Andrew Chou, Laurence Chen
Outreach lesson Andrew Chou
Grade specific lessons Stephen Chiang, Sandra Chang, Ted Chueh, Keelan Tseng
Education Workshops Terry Cheng, Charlotte Hsu, Andrew Chou, Laurence Chen, Phoebe Lin

Wiki and Deliverables

Classifiction Name
Logo design and art Kate Wang
Visual design and presentation Kate Wang
Wiki coding and programming Luigi Wang, Stephen Chiang, Sandra Chang, Ted Chueh, Keelan Tseng
Animations Kate Wang
Main presentation Victoria Wang, Phoebe Lin, Charlotte Hsu, Terry Cheng
Wiki content Felix Tsai, Grace Chang, Kai Hsiao, Terry Cheng, Charlotte Hsu, Andrew Chou, Victoria Wang, Hao-Hung Chen, Lira Lee
Photos Andrew Chou

Collaborators
iGEM_ASIJ
iGEM Korea_HS
iGEM NYCU_Formosa
iGEM GEMS_Taiwan
iGEM Empire Gene
iGEM Taipei Wego
iGEM TEC Chihuahua
iGEM Alumni: iGEM Exchange Conference
Kang Chiao International School Synthetic Biology Class 2023

Experts

  • Dr. Fang-Jen Lee from College of Medicine, NTU supplied us with several plasmids for years and also helped our lab set up an account at the human disease modeling center in the NTU for us to get yeast knockout gene mutant strains.

  • Dr. Chen Shao-Kuan from Institute of Neuroscience, NCCU, collaborated with us so team members can go to his lab for summer and winter break internship. He also provided us with advice regarding the metabolism due to his familiarity with metabolism as he is working on the same gene in cancer cells as our project is.

  • Dr. Pei-Wen Chu , Principal Research Scientist from Chimera Bioscience Inc., guided us with improvements about cloning sequencing and experimental designs.

  • Dr. Tien Hsien, Chang from Genomics Research Center, Academia Sinica and Dr. Yi-Chun Wu Lab from the National Taiwan University supplied us with plasmids in bacteria and also yeast for the project alongside several suggestions regarding the materials in the experiment.

  • Dr. Hsiao-Fen Han, Ph.D, Postdoctoral Research Fellow, provided constructive feedback about plasmids and enzymes in our experimental designs.

    • Wiki Support

  • Ms. Lali Curry was our Wiki Page Consultant, helping students to understand the coding and uploading of the Wiki Page.

    • Advisors

  • Tarn Parker was the primary Principal Investigator of our team. She was in charge of the entire team throughout the competition. As a position of authority, she planned out our project schedule as well as the wiki page designing schedule and running meetings with the team, and between the team and various instructors.

  • HuiChing Kuo was the instructor. She was responsible for giving lectures about the basics of synthetic biology followed by the process of many different synthetic biology protocols. She also created the backbones of multiple protocols such as the overexpression of genes and making yeasts then guided us through the process.

  • Wan-Syuan Lin was the secondary Principal Investigator. As an assistant to the members and project, she purchased lab materials, and dealt with the school affairs. She made sure the completion of the course and supplies were as smooth as possible.

  • Chloe Sheu's previous experience on an iGEM team was invaluable to helping us. She provided key support in making connections with other teams for the collaborations that we formed, as well as helping us to maintain focus on the key requirements of the project.

  • Sabrina Yeo, a Taiwan iGEM ambassador, provided us with valuable advice and direction in navigating the project for the first time.

    • Access Wiki Tools View Source Code