Attributions

Principal Investigator

Dr. Saravanan Palani - (Department of Biochemistry, IISc) helped us in experiment design, cloning, and protein production strategies. He allowed us unfettered access to his laboratory for this purpose. He gave us the freedom to attempt novel approaches while keeping us strongly grounded in ethics.

Advisor

Anubhav Dhar - A graduate student at SP lab, he provided unwavering support at each stage of the experiment. He assisted in troubleshooting our biology lab work.

Team

Student Leader

Megha Kattimani - Responsible for directing the team throughout the project and ensuring cooperation across different project components. Participated in the project's brainstorming and planning phases. In addition to contributing to the promotional video and wiki content, she was in charge of Drylab project management, Public Education and Engagement, and IHP initiatives. Moreover, she was in charge of all official clearances, financial management, and administrative requirements.

Student Deputy Leader

Ayushi Gupta - Co-led the team and enabled cooperation across various project components. She was a part of the brainstorming and planning of the project's general strategy. Along with leading education, communication, IHP initiatives, and contributing to the wiki, she was also involved with wet lab work and was in charge of all administrative needs as well. Diplomatically handled all affairs. Did not shy away from performing smaller tasks.

Wetlab

Coordinators

Kredai Raaman - In charge of wet lab work and design of experiments, including in-silico tests. Was helpful in identifying problems in the wet lab that required answers via the Drylab team’s Modelling. Assisted in scientific aspects of the bioreactor.
Siddharth Bothra - Was in charge of wet lab work. He was also active in public engagement, education, brainstorming the bioreactor design, and connecting the bridge between the wet lab and dry lab. His constant support of establishing cooperation between different segments of the project, his contribution was crucial to the team's functioning.

Members

Jeevan Subodh B - Worked in wet Lab, was involved in brainstorming and worked on translating material to Kannada.
Soham Mahesh Karandikar - Dedicatedly worked for wet lab, his hands magically fixed experiments when prospects seemed bleak.
Divija Nanavati - Worked on inter-lab experiments and was part of educational activities.
Anjaney Jaishankar Prasad Pandey - Worked on inter-lab experiments and content writing for educational material.
Oviya - Worked on inter-lab experiments, and content writing for educational material and was also actively involved in the design activities of the project.

Drylab

Coordinators

Aryaman Bhutani - Responsible for DryLab activities, coordinated dry lab and design of the bioreactor, was involved in wiki development and design activities and was prominently involved in the making of the project promotion video. A social media manager, and a constant cheerleader for members working in the wet lab, he also volunteered for human practices and IHP activities. Was actively involved in the structural modeling related to our project.
Krishna S Bharadwaj - Responsible for DryLab activities, coordinated dry lab and the design of the bioreactor, was involved in wiki development and design activities, and was actively involved in the design, documentation and performing the chemistry lab experiments. He was involved in the enzyme kinetics modelling and was involved in the brain storming from the beginning.

Members

Soumyadeep Sarma - He has been involved in the modelling and simulation of the enzyme model, fianacial modelling and website. He has also volunteered for IHP activities.
Aviral Sood - He has been involved in the modelling and simulation of the enzyme model and fianacial modelling. He has also volunteered for IHP activities.
Keshav Agarwal - He was involved in Drylab brainstorming, content writing for education, and content proofreading. Constantly supported teammates' mental health. Fixed logistical issues that were raised.
Prithu Purkait - He was involved in verifying and brainstorming models for the project, especially in drylab and hardware.

Bioreactor

Shreshth Jaiswal - Apart from working actively on the design of the bioreactor, he was involved in the direction of the project promotion video, animating several portions of the video and editing. He is also talented in web development, and worked on building the wiki and miscellaneous design activities. Designed the bioreactor model. He was also active in the brainstorming phase of the project. He was involved in IHP activities as well.
Dhruv Gupta - The initial idea, Halocleen, was conceived by him, and he dedicated himself in the brainstorming phase. He can be called an idea machine, as he can come up with many creative ways to come up with solutions to problems. He was keen on developing the most suitable bioreactor for our project. He was also involved in IHP activities, modifying the bioreactor continuously in response to inputs from experts.

Chem Lab

Coordinator

Nakul Abhay Bapat - Not only a chemistry wizard but also a talented graphic designer who designed the current logo of our project. He made some of the beautiful designs you see related to our project. He was actively involved in the brainstorming phase of our project and dedicated himself to developing the current idea. Performed chemical analysis related to our project.

Members

Dev Dodiya - A chemistry enthusiast, worked on chemical analyses related to our project.
Pranav Rao - Another chemistry enthusiast who conducted experiments in the chemistry lab.

Human Practices and Education

Coordinators

Souvik Mandal - Words are inadequate to describe his ability to brainstorm for human practices activities related to our project. He was tirelessly involved in developing strategies for all our educational activities and pushed the team to pursue several ambitious activities.
Sharwary M R - The most patient coordinator. She handled various activities regarding education and communication aspects of the project. Came up with creative ways to educate children. Documented all the activities meticulously. Also, an ecology enthusiast who gave an environmental perspective while making educational material.

Members

Sanmit Chakraborty - He performed the crucial task of coordinating logistics for the All India iGEM Meet. Was involved in the translation of the education material to Bengali. He was also involved in the activities of the education team.
Diya Sarkar - She supported all the educational activities and was involved in the inter-lab study experiments. She was actively involved in the Bengali translation of the project promotion video, and an active content team member. Was involved in developing the Educational Material.
Debadrito Roy - An education enthusiast, he brainstormed educational activities and Human Practices activities. A volunteer who helped in several logistical activities. He was actively involved in logistics execution during the All India iGEM Meet. Socialized with our fellow teams.
Parth Deshpande - He was involved in several communication activities. A talented Marathi translator, he was actively involved in producing our project promotion video.
Atharv Suryawanshi - Worked for the educational pursuits of our team. He was also involved during the initial brainstorming of our bioreactor and was involved in biosafety design for our bioreactor.
Vinishaa Sree - Worked for the educational pursuits of our team.
Aramaan Meher - Volunteered for AIIM and the educational activities

Content and Design

Coordinators

Saakshi Porwal - This vocabulary goddess handled all our team's content development activities. Hosted the events during the All India iGEM Meet. Proofread most of the content you are reading related to our project. She was also actively involved in the production of the project promotion video.

Member

Sudhanva - A dedicated content wizard. Proofread and translated content, and participated in our team's educational and outreach activities.
Shinjini - Was involved in content writing, proofreading, and animating the project promotional video.

Website

Coordinator

Jasnoor Singh - Our saviour, coordinated all the activities of the website production

Member

Shreenabh Agrawal - Worked on uploading content to our website

Integrated Human Practices

Jyotirmoy Biswal - Helped with brainstorming for activities for education. Actively involved in logistics and organization of All India iGEM Meet.
Sanket Patil - Worked on activities related to Integrated Human Practices

Mentor

Sukriyo Chakraborty
He is a person who is difficult to impress, ex-team leader of iGEM IISc. His high standards and perfectionism inspired all of us. Guided us at every stage of our project. His inputs were invaluable to us.

Additional Support

WET LAB SUPPORT

Anubhav Dhar, Bindu Bhojappa, Nishaant P.B., and the rest of the members of the Saravanan Palani Lab (Department of Biochemistry, Indian Institute of Science) for technical assistance and guidance regarding wet lab work, and Penchalayya (Lab Technician, Saravanan Palani lab)
Dr. A.T. Biju (Associate Professor, Department of Organic Chemistry, Indian Institute of Science) for providing us access to his lab and the Gas Chromatography facility and Avishek Guin (A.T. Biju lab, Indian Institute of Science) for technical assistance with the Gas Chromatography instrument
Dr. Nagasuma Chandra (Professor, Department of Biochemistry, Indian Institute of Science) for providing us access to her BSL2 facility and the members of Nagasuma Chandra Lab
Dr. Ramray Bhat and Dr. SP Arun, the UG Biology Coordinators, for allowing us access to the UG Biology laboratory for meetings and for wet lab work
Vinod H.S. and Deepthi Ravi (Teaching Assistants, UG Biology lab, Indian Institute of Science), for training us in basic wet lab techniques and technical assistance

TECHNICAL HELP

Dr. Debasis Das (Assistant Professor, Department of Inorganic and Physical Chemistry, IISc), for assistance with understanding the enzyme systems under consideration
Dr. Dipankar Chatterjee (Professor, Molecular Biophysics Unit, IISc) and Dr. Rajasree K. P. (Umesh Varshney Lab, IISc), for assistance with plasmid design and cloning
Dr. Umesh Varshney (Professor, Department of Microbiology and Cell Biology, IISc), for assistance with troubleshooting the cloning process
Dr. Sumantra Sarkar (Assistant Professor, Department of Physics, IISc), for helping us understand how to apply physical principles to model biological systems
Dr. Mohit Kumar Jolly, (Assistant Professor, Department of BioSystems Science and Engineering, IISc), for guidance regarding modelling systems and using experimental data to refine our models
Dr. Ishaan Gupta (Indian Institute of Technology – Delhi), for technical assistance regarding bioreactors and assistance in adapting our proposal to an airlift design
Dr. G.K. Suraishkumar (Indian Institute of Technology – Madras) and Sonal Kumar, for identifying flaws in the RBC design and assistance in constructing the final Solid State Design model
Dr. Shishir (Robert Bosch Centre, IISc), for suggestions regarding implementation
Dr. Dipshika Chakravortty, (Professor, Department of Biochemistry, IISc), and Dr. Samay Pande (Assistant Professor, Department of Microbiology and Cell Biology, IISc) for assistance during the initial brainstorming phase
Nikhita Kirtivasan, Aditya Basu, Mohit Das, Bharat Vivan, and Lakshya Chauhan, (Undergraduate Program, IISc), for assistance in refining our project during the brainstorming phase

MATERIAL SUPPORT

Dr. Prashant Phale (Professor, Indian Institute of Technology – Bombay), for gifting us Pseudomonas putida KT2440
The Standard European Vector Architecture repository in Madrid for gifting us cloning vectors for Pseudomonas putida
Dr. Teruyuki Nagamune (Professor, University of Tokyo) and the AddGene repository, for providing us the pP40cam, pPdR, pPdX, and pBB542 plasmids
Melanie Allegaier and Dr. Julian Wissner (University of Stuttgart), for gifting us the pBAD19 plasmid bearing TodC1C2AB
Andrew Crombie and Dr. J. Colin Murrell (Director of the Earth & Life Systems Alliance, University of East Anglia), for gifting us Methylocella silvestris BL2 genomic DNA.
A.T. Biju Lab, Utpal Tatu Lab, P.N. Rangarajan Lab, Debabrata Laha Lab, Umesh Varshney Lab, Purusharth Rajyaguru Lab, Dipshika Chakravortty Lab, and K. Muniyappa Lab (Division of Biological Sciences, Indian Institute of Science) for material assistance

PUBLIC ENGAGEMENT

Dr. Bhaskar Kura (University of Orleans) for helping us analyze our sustainability goals.
Mr. Ian Crosby (Former Manager of Sustainability Initiatives in East Asia and the Pacific) for helping us determine our entrepreneurial position.
Mr. Unmesh Patil (Member of Parliament, India), Ms. Anja Werntges, and Ms. Irene Papst (Deutsche Gesellschaft für Internationale Zusammenarbeit) for providing insight about action of the authorities on the subject of halocarbon degradation.
Mr. Rajesh Patil (Commissioner Municipal Corporation), Dr. Bitasta Das (Senior Editor, Indian Institute of Science), Ms. Varsha Jaisimha (Graduate Student, Imperial College; iGEM Alumni) for helping us pan out stakeholders and identify potential audience for education.
Tsungrojungla Walling, Barnopriyo Dutta, Dipayan Datta, Shreeya Moghe, Aatman Supkar (Undergraduates, IISc) for design, content and promotion video.
Anurag Chittawar, (Undergraduate, IISc) for helping us with brainstorming session.
Senin Ahammed A P, Archana Porwal for translating 'Sync' to different languages.
Team iGEM IIT-Delhi and team iGEM IISER-Pune for education and dry-lab execution.
Ms. Pratishtha (Prayoga Institute for Education Research), Mrs. Chitra Kattimani, Dr. Susmita Panda, Mr. SM Kattimani for reviewing our educational material and suggesting edits.
Dr. HS Nagaraja (Director, Prayoga), Mr. Ramachandra R, Mr. Chennappa for helping us establishing contacts and executing our activities at a larger level.
Mr. Srigopal Khedia, Mr. Avishek Mukherjee (Merchant Navy) for providing us information on cooling on vessels.

Sponsors

We thank our esteemed sponsor: Indian Institute of Science, Bengaluru for providing major monetary support which was indispensable for our project!
We would also like to extend a heartfelt thanks to SnapGene, Integrated DNA Technologies!
We are also grateful to Promega for recognising our project and granting us a voucher of 2500 USD worth of products from Promega.
Extended thanks to Students' Council IISc, Promega for the monetary support, and Coca Cola for providing beverages for the event of AIIM 2022.