Section 1 : Introduction
This web page is dedicated to everyone who has contributed to and supports our project.
Section 2 : Laboratory
Designer: Daining Gu, Xinmin Liu
Monitor and leader: Zhichao Li, Yuanhan Sui, Yue Wu
Participants of plasmid construction experiment: Ziqi Xie, Yiping He, Xingyi Wen, Aobo Sun, Tianhao Huang, Ranting Zhao, Kaixin Zhan, Jiayi Li, Yundi Chen, Heng Zhang.
Participants of protein expression experiment: Jiayi Li, Ziqi Xie, Xingyi Wen, Kaixin Zhan.
Daining Gu, Yuanhan Sui and Zhichao Li measured the Tb3+ biosorption by engineered strains and adhesion ability of engineered cells to silica particle, which provided data for constructing models.
Section 3 : Modeling and Analysis
Leader: Da Zheng
Work: Prediction of the capture protein with Robetta Server and optimization. Verification of the protein’s structure. Validation of the function of the fusion protein. Construction of mathematic models, basic model and diffusion model.
Function of these models: Mathematic models enabled us to understand deeply of the expression process and work principle. Basic model helped to decipher the function of sensing part whose results validated the success of our molecular biology design. Diffusion model made it possible to apply REE Miner to dry lab in the future.
Section 4 : Hardware
Leader: Zuoming Fu
Hardware: Being inspired by the distillation tower——we built our hardware, a layered long barrel structure with each layer as a separator. And we add a sterilization chamber at the exit. The barrel is filled with silicon dioxide and our engineering bacteria to better recover REE. When the miner wastewater flows into the column with the buttons closed, the REE can be captured. And after several hours, we can open the buttons to fetch water lacking REE, leaving the REE in the column.
Section 5 : Wiki
Leader: Guanyao Cheng, Da Zheng
Construction: Daining Gu, Yuanhan Sui, Yue WU
Participants: all members
Section 6 : Human practice
Leader: Chongzhe Tian, Heng Zhang
Work: activities of integated human practice, operation QQ acount of HUST-China, interviews with relevant stakeholders, conducting, field research, samples mine wastewater.
Participants: Xingyi Wen, Ranting Zhao
Section 7 : Education & Communication & Collaborations
Education
Leader: Xuran Chen
Tasks: science lecture in Wuhan science and technology museum, science courses in high school students’ summer camp
Planner of science lecture: Yiping He
Participants: Chongzhe Tian, Xuran Chen, Ranting Zhao, Xingyi Wen, Jiayi Li, Ziqi Xie, Daining Gu, Zhichao Li, Zhengkai Zhuang, Da Zheng
Communication and collaborations:
Tasks: Central China Conference, communication with China University of Geosciences.
Participants: Xuran Chen and Ranting Zhao
Section 8 : Creative work
Leader: Mingyuan Cheng
Main designer: Xiyuan Zhang, Shengbiao Wu
Products: team logo, uniform, art design of wiki
Section 9 : Video
Director: Zhengkai Zhuang
Dubbing: Jiayi Li, Xuran Chen, Yuanhan Sui
Script and scene: Xiyuan Zhang
Shooting and editing: Chongzhe Tian, Xiyuan Zhang, Shengbiao Wu, Xingyi Wen, Xinmin Liu.
Section 10 : Support
Many thanks for guidance and advice we received from our PIs and professors we interviewed. Without them we would not be able to complete such a successful project.
Experiment support
Shangxian XieWe consulted him some questions in experiment design and got some useful advice.
Bohan Wang Changxin Fan Jiacheng ShiThey were student leaders in 2021 HUST-China and gave us some helpful suggestions in many aspects.
Model support
Letian GaoHe gave us a hand to construct wiki and offered help to us in modeling.
Lab support
Mengqu RanAs our safety officer, she taught us the lab rules and guaranteed our safety. Also, she told us some experience in the lab about experiment.
Zhen Zheng(left) Shuhan Dai(middle) Qing Chen(right)They were our teachers in the lab who taught us the methods of operating the experiment instruments and some useful experiment operation skills.
Other support
Guofan ZhangProfessor Zhang provided some academic suggestions for us.
Chaoran Lin & Depeng LiuThey are engineers in Longyan rare Earth Development Co.LTD who provided us with some information of sewage treatment in their company which was helpful for us to put our products into use.
Chaoran Liu(left) Tiankai Dai(right)As the member of 2021 HUST-China, Tiankai Dai and Chaoran Liu helpd us finish the online teaching video and modify some PowerPoints for us.
Ye ChenHe provided us with some wonderful photos.