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Section 1 : Introduction

This web page is dedicated to everyone who has contributed to and supports our project.

Section 2 : Laboratory

Designer: Daining Gu, Xinmin Liu

Monitor and leader: Zhichao Li, Yuanhan Sui, Yue Wu

Participants of plasmid construction experiment: Ziqi Xie, Yiping He, Xingyi Wen, Aobo Sun, Tianhao Huang, Ranting Zhao, Kaixin Zhan, Jiayi Li, Yundi Chen, Heng Zhang.

Participants of protein expression experiment: Jiayi Li, Ziqi Xie, Xingyi Wen, Kaixin Zhan.

Daining Gu, Yuanhan Sui and Zhichao Li measured the Tb3+ biosorption by engineered strains and adhesion ability of engineered cells to silica particle, which provided data for constructing models.

Section 3 : Modeling and Analysis

Leader: Da Zheng

Work: Prediction of the capture protein with Robetta Server and optimization. Verification of the protein’s structure. Validation of the function of the fusion protein. Construction of mathematic models, basic model and diffusion model.

Function of these models: Mathematic models enabled us to understand deeply of the expression process and work principle. Basic model helped to decipher the function of sensing part whose results validated the success of our molecular biology design. Diffusion model made it possible to apply REE Miner to dry lab in the future.

Section 4 : Hardware

Leader: Zuoming Fu

Hardware: Being inspired by the distillation tower——we built our hardware, a layered long barrel structure with each layer as a separator. And we add a sterilization chamber at the exit. The barrel is filled with silicon dioxide and our engineering bacteria to better recover REE. When the miner wastewater flows into the column with the buttons closed, the REE can be captured. And after several hours, we can open the buttons to fetch water lacking REE, leaving the REE in the column.

Section 5 : Wiki

Leader: Guanyao Cheng, Da Zheng

Construction: Daining Gu, Yuanhan Sui, Yue WU

Participants: all members

Section 6 : Human practice

Leader: Chongzhe Tian, Heng Zhang

Work: activities of integated human practice, operation QQ acount of HUST-China, interviews with relevant stakeholders, conducting, field research, samples mine wastewater.

Participants: Xingyi Wen, Ranting Zhao

Section 7 : Education & Communication & Collaborations

Education

Leader: Xuran Chen

Tasks: science lecture in Wuhan science and technology museum, science courses in high school students’ summer camp

Planner of science lecture: Yiping He

Participants: Chongzhe Tian, Xuran Chen, Ranting Zhao, Xingyi Wen, Jiayi Li, Ziqi Xie, Daining Gu, Zhichao Li, Zhengkai Zhuang, Da Zheng

Communication and collaborations:

Tasks: Central China Conference, communication with China University of Geosciences.

Participants: Xuran Chen and Ranting Zhao

Section 8 : Creative work

Leader: Mingyuan Cheng

Main designer: Xiyuan Zhang, Shengbiao Wu

Products: team logo, uniform, art design of wiki

Section 9 : Video

Director: Zhengkai Zhuang

Dubbing: Jiayi Li, Xuran Chen, Yuanhan Sui

Script and scene: Xiyuan Zhang

Shooting and editing: Chongzhe Tian, Xiyuan Zhang, Shengbiao Wu, Xingyi Wen, Xinmin Liu.

Section 10 : Support

Many thanks for guidance and advice we received from our PIs and professors we interviewed. Without them we would not be able to complete such a successful project.

Experiment support

Shangxian Xie

We consulted him some questions in experiment design and got some useful advice.

Bohan Wang Changxin Fan Jiacheng Shi

They were student leaders in 2021 HUST-China and gave us some helpful suggestions in many aspects.

Model support

Letian Gao

He gave us a hand to construct wiki and offered help to us in modeling.

Lab support

Mengqu Ran

As our safety officer, she taught us the lab rules and guaranteed our safety. Also, she told us some experience in the lab about experiment.

Zhen Zheng(left) Shuhan Dai(middle) Qing Chen(right)

They were our teachers in the lab who taught us the methods of operating the experiment instruments and some useful experiment operation skills.

Other support

Guofan Zhang

Professor Zhang provided some academic suggestions for us.

Chaoran Lin & Depeng Liu

They are engineers in Longyan rare Earth Development Co.LTD who provided us with some information of sewage treatment in their company which was helpful for us to put our products into use.

Chaoran Liu(left) Tiankai Dai(right)

As the member of 2021 HUST-China, Tiankai Dai and Chaoran Liu helpd us finish the online teaching video and modify some PowerPoints for us.

Ye Chen

He provided us with some wonderful photos.

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