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Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts
The below parts are used in our project. A description is provided for each designed part.
This protein is expected to bind to valerolactam. It is modified by our team from NitR engineered for ε-caprolactam or valerolactam biosynthesis from 6-ACA or 5- AVA. This protein is found present in Alcaligenes faecalis.[1]
This protein is expected to bind to valerolactam. It is modified by our team by mutating the 117th residue leucine to phenylalanine. We had modified the protein as it was reported that highly sensitive nitR-L117F can be used for the ε-caprolactam biosynthesis pathway.[1]
This protein is expected to bind to valerolactam. It is modified by our team by mutating the 117th residue leucine to Serine. We had modified the protein as it was reported that L117S is a mutant of nitR which is the most sensitive to ε-caprolactam. This protein is found present in Alcaligenes faecalis.[1]
This protein is expected to bind to valerolactam. It is modified by our team by mutating the 117th residue leucine to Tryptophan. We had modified the protein as it was reported that L117W is a mutant of nitR which is the second most sensitive to ε-caprolactam. This protein is found present in Alcaligenes faecalis.[1]
This protein is expected to bind to valerolactam. It is the original cyclase. We have chosen to use the protein as it was reported that nitR_WT is used for the highly specific detection of lactam compounds against lactam biosynthetic intermediates. This protein is found present in Alcaligenes faecalis.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. It is the wild type cyclase. We have chosen to use this cyclase as it was reported that an ORF encoding Cf3hbd from Citrobacter freundii (CF3HBD) has a positive fluorescence signal corresponding to the conversion of 6-ACA to ε-caprolactam, which indicates that it may also binds to 5AVA and convert to valerolactam.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue from Tyrosine to Alanine. We have modified the cyclase as it was reported that Y152A mutant showed the highest cyclization activity, with a three-fold increase, for 6-ACA, which indicates that it may also show the highest cyclization activity for 5AVA. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Glutamic acid, and the 184th residue Tryptophan to Phenylalanine. We had modified the cyclase as it was reported that Y152E has the high specific activity of 28.5 ± 1.3 U/mg of detecting ω-amino fatty acids, such as 5-AVA and 6-ACA, via lactam formation. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Glutamic acid, and the 184th residue Tryptophan to Tyrosine. We had modified the cyclase as it was reported that Y152E has the high specific activity of 28.5 ± 1.3 U/mg of detecting ω-amino fatty acids, such as 5-AVA and 6-ACA, via lactam formation. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Glutamic acid. We had modified the cyclase as it was reported that Y152E has the high specific activity of 28.5 ± 1.3 U/mg of detecting ω-amino fatty acids, such as 5-AVA and 6-ACA, via lactam formation. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Valine, and the 184th residue Tryptophan to Phenylalanine. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Valine, and the 184th residue Tryptophan to Tyrosine. This cyclase is found present in Citrobacter freundii.[1]
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue Tyrosine to Valine. This cyclase is found present in Citrobacter freundii.[1]
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K4384000 | Coding | nitR_L117F | Young Hong Yui | 984 |
| BBa_K4384001 | Coding | nitR_L117S | Young Hong Yui | 984 |
| BBa_K4384002 | Coding | nitR_L117W | Young Hong Yui | 984 |
| BBa_K4384003 | Coding | nitR_WT | Young Hong Yui | 984 |
| BBa_K4384004 | Coding | 5YSS_WT | Young Hong Yui | 771 |
| BBa_K4384005 | Coding | 5YSS_Y152A | Young Hong Yui | 771 |
| BBa_K4384006 | Coding | 5YSS_Y152E_W184F | Young Hong Yui | 771 |
| BBa_K4384007 | Coding | 5YSS_Y152E_W184Y | Young Hong Yui | 771 |
| BBa_K4384008 | Coding | 5YSS_Y152E | Young Hong Yui | 771 |
| BBa_K4384009 | Coding | 5YSS_Y152V_W184F | Young Hong Yui | 771 |
| BBa_K4384010 | Coding | 5YSS_Y152V_W184Y | Young Hong Yui | 771 |
| BBa_K4384011 | Coding | 5YSS_Y152V | Young Hong Yui | 771 |