Utilized and Characterized Existing Parts
| Part name | Part number | Contribution |
|---|---|---|
| Heavy chain human ferritin | BBa_K1189025 | To recycle our enzyme-expressing microbes and cellulosome complexes after the degradation of fabric, magnetism is the key point. Utilizing the human ferritin heavy chain provided by iGEM13_Calgary, we are able to generate magnetoferritin via the chemical modification of the original design - the addition of ammonium iron (II) sulfate into the medium. |
| eforRed, red chromoprotein | BBa_K592012 | Proving the functionality of our giant cellulosome complex is indispensable for our project. The eforRED chromoprotein provided by Uppsala-Sweden is the best candidate for our reporter protein. We developed 3 different fusion variants of eforRED, each testing for a specific molecular interaction in our construct. The bright color and high expression level of eforRED facilitate our observation and data collection to a great extent. |
| PETase-5, PET hydrolase | BBa_K3715005 | The degradation of polyester fiber is a crucial part of the project FabRevivo. Due to the low catalytic efficiency and thermostability of the wild-type PETase, we craved a highly efficient PETase mutant that can degrade high-crystallinity polyester fiber under high temperatures. Thanks to the Super-5 PETase mutant provided by TJUSLS_China, the degradation of this recalcitrant artificial fiber will no longer be a time-consuming process. Furthermore, we developed a Dockerin-fused variant of this miraculous PETase mutant. |
New Basic Parts
| Part name | Part number | Sequence correct? | Group favorite |
|---|---|---|---|
| Kmarx Mα | BBa_K4275000 | Yes | |
| C1 ori | BBa_K4275001 | Yes | |
| NpaBGS-T | BBa_K4275002 | Yes | |
| TaLPMO-t | BBa_K4275003 | Yes | |
| CBHII-T | BBa_K4275004 | Yes | |
| MtCDH-t | BBa_K4275005 | Yes | |
| TrEGIII-t | BBa_K4275006 | Yes | |
| MHETase | BBa_K4275009 | Yes | |
| MHETase-t | BBa_K4275010 | Yes | |
| PETase5-dockerin | BBa_K4275011 | Yes | |
| CipA1B2C | BBa_K4275012 | Yes | 1 |
| OlpB3CAg3 | BBa_K4275013 | Yes | 11111 |
| Neae-Nb3 | BBa_K4275026 | Yes | |
| eforRed-Ag3 | BBa_K4275027 | Yes | |
| eforRed-dockerin i | BBa_K4275028 | Yes | |
| efored-dockerin ii | BBa_K4275029 | Yes | |
| pRha-riboj | BBa_K4275030 | Yes | 1 |
| PfuFerritin-wt | BBa_K4275031 | Yes | |
| PfuFerritin-Nb3 | BBa_K4275032 | Yes | |
| K.marxianus pLAC4 | BBa_K4275051 | Yes |
Composite Parts
| Part name | Part number | Composite of: | Sequence correct? | Group favorite |
|---|---|---|---|---|
| pLac4-Kmarx Mα-TaLPMO-t-tTDH1 | BBa_K4275037 | K4275050+ K4275000+ K4275003+ K2753052 | Yes | |
| pLac4-Kmarx Mα-NapBGS-T-tTDH1 | BBa_K4275036 | K4275050+ K4275000+ K4275002+ K2753052 | Yes | |
| pLac4-Kmarx Mα-CBHII-T-tTDH1 | BBa_K4275038 | K4275050+ K4275000+ K4275004+ K2753052 | Yes | |
| pLac4-Kmarx Mα-MtCDH-t-tTDH1 | BBa_K4275039 | K4275050+ K4275000+ K4275005+ K2753052 | Yes | |
| pLac4-Kmarx Mα-TrEGIII-t-tTDH1 | BBa_K4275040 | K4275050+ K4275000+ K4275006+ K2753052 | Yes | |
| pT7-CipA1B2C-tT7 | BBa_K4275041 | BBa_J64997+BBa_K3715082+ BBa_K4275012+BBa_K731721 | Yes | 11111 |
| pT7-OlpB-Ag3-tT7 | BBa_K4275042 | BBa_J64997+BBa_K3715082+ BBa_K4275013+BBa_K731721 | Yes | 1 |