2022 GDSYZX IGEM project presentation¶
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Patchoulol or patchouli alcohol (C15H26O) is a sesquiterpene alcohol found in patchouli. Patchouli oil is an important material in perfumery. The (−)-optical isomer is one of the organic compounds responsible for the typical patchouli scent. Patchoulol is also used in the synthesis of the chemotherapy drug Taxol.
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Title: Application of photoinducible promoter in the biosynthesis of patchoulol in yeast
Abstract: Patchoulol is one of the main active components of Pogostemon cablin, and it is also an important indicator for evaluating the medicinal materials of P. cablin in the Chinese Pharmacopoeia. Patchoulol can be obtained by natural extraction, chemical synthesis, and synthetic biology engineering. Due to the low content of patchoulol in P. cablin and the low yield of chemical synthesis, the study of combinatorial biosynthesis and molecular regulation can provide the basis for obtaining high-content patchoulol. In this study, on the basis of the biosynthetic pathway, molecular regulation mechanism and synthetic biology engineering of patchoulol, the key genes for its biosynthesis were combined and expressed in heterologous host yeast to produce patchoulol, which provides the technical foundations of the development and application of patchoulol.
Keywords: Patchoulol; Pogostemon cablin; sesquiterpene; constitutive promoter; photoinducible promoter;