Future Directions

Experimental Validation


Currently, our system only displays oxybenzone levels using light intensity and is analyzed using fluorescence microscopy which limits the scope of our project. In order to better quantify our data, we wish to create a tie-in computer program, involving machine learning, that is able to provide easily quantifiable and modifiable online data to the scientist. They can then use the data to develop stronger conclusions and conduct more in-depth research. In addition to this, there is no particular threshold of oxybenzone concentration that causes animal and human harm. This means that the more oxybenzone present, the greater the damage. Armed with these facts, we would design our system to be used to help gain the data needed to develop oxybenzone threat thresholds. As a result it would help scientists and policymakers understand the effects of oxybenzone on coral reefs. Also, the existence of an oxybenzone threshold level could help make our product more useful for scientists who would be able to easily audit a location for the presence of harmful levels of oxybenzone. Though we have developed a thorough model for our project, we recognize that we were unable to access a wet lab this year, so further testing will be needed to make sure our system functions well in the intended setting.

Implementation


Our device represents a novel, in-situ method of testing for the presence of oxybenzone. This system will assist scientists who work with water samples taken from the source. They simply have to load the device with the water samples and view the results using light intensity. Since our device is portable, scientists will be able to immediately see test results, particularly helping scientists working in remote locations and without immediate access to a lab. Prior to the creation of our method, scientists would have had to collect samples and wait to reach a stable lab before conducting tests. However, with our solution, they could conduct experiments and get fast results anywhere and anytime in the world. This method is also non-invasive to marine ecosystems, as only a small sample of water will need to be taken. In the future, we look to expand our device to locations such as beaches, resorts, and pools where oxybenzone-containing sunscreens are widely used. The owners of these locations would be able to measure the concentration of oxybenzone – a chemical harmful to the human endocrine system. They could then take action to preserve their patrons’ health by consistently replacing the pool water or banning the use of oxybenzone sunscreen.

Another benefit would be the monitoring of marine environments. Scientists will be able to monitor the oxybenzone levels in coral reefs. This could help prevent harm to marine ecosystems, allowing for the continued appreciation and maintenance of the world’s biodiversity.

Finally, another possible avenue for growth is the continued measurement of oxybenzone levels over long periods of time. These could be set up throughout the world and connected to the cloud for easy access for scientists. Replaceable nutrition cartridges for the bacteria could help reduce the needed maintenance, making it easy for scientists to gain valuable insights through these systems.

Testing Concerns


It is crucial to consider the efforts needed to create the modified E. coli bacteria. We must also note the amount of acceptance within the scientific research community and the various environmental concerns in the status quo. Bacteria related safety concerns can be addressed by adding guidance and materials for safe disposal. For example, bleach or other chemicals could be added to field kits for use to avoid bacteria leaks during disposal.

References


Scientist taking water samples. Scientist Taking Water Samples | U.S. Geological Survey. (n.d.). Retrieved October 10, 2022, from https://www.usgs.gov/media/images/scientist-taking-water-samples