Parts
Yeast (Group Y) Parts
All genetic parts of group Y can be divided into 3 categories, including single element, key composite segment, and vector.
Group Y single elements
The single elements refer to every functional unit of our key genetic parts, like promoter, linker, important polypeptide, etc. A total of seven single elements were documented.
| Part Code | Part name | Link |
|---|---|---|
| BBa_K4161500 | 20ipaD for S. cereviae Expression | http://parts.igem.org/Part:BBa_K4161500 |
| BBa_K4161501 | T7 Promoter | http://parts.igem.org/Part:BBa_K4161501 |
| BBa_K4161502 | GAL1 Promoter | http://parts.igem.org/Part:BBa_K4161502 |
| BBa_K4161503 | Aga2 | http://parts.igem.org/Part:BBa_K4161503 |
| BBa_K4161504 | G4S | http://parts.igem.org/Part:BBa_K4161504 |
| BBa_K4161505 | T7, Xpress | http://parts.igem.org/Part:BBa_K4161505 |
| BBa_K4161506 | ipaD | http://parts.igem.org/Part:BBa_K4161506 |
| BBa_K4161507 | HA | http://parts.igem.org/Part:BBa_K4161507 |
Group Y key composite segments
The key composite segments are clusters of multiple single elements, they make up the key expression modules or functional units of the genetic model. Two key composite segments were documented.
| Part Code | Part name | Link |
|---|---|---|
| BBa_K4161900 | Single Domain Antibody 20ipaD Conjugated Aga2 Express Module | http://parts.igem.org/Part:BBa_K4161900 |
| BBa_K4161508 | 488aa recombinant protein, Aga2 + ipaD | http://parts.igem.org/Part:BBa_K4161508 |
Group Y vector
Most of our genetic designs were based on the yeast surface display vector pYD1, which is also documented.
| Part Code | Part name | Link |
|---|---|---|
| BBa_K4161302 | pYD1 plasmid | http://parts.igem.org/Part:BBa_K4161302 |
E.coli Nissle 1917 (Group E) Parts
Group E single elements
Single elements of Group E include nanobodies, curli protein (csg) operon fragments, linkers, tags, signal peptides, promoters and terminators. A total of 15 parts were documented.
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161001 | 20IpaD | http://parts.igem.org/Part:BBa_K4161001 |
| BBa_K4161002 | JMK-H2 | http://parts.igem.org/Part:BBa_K4161002 |
| BBa_K4161010 | csgA | http://parts.igem.org/Part:BBa_K4161010 |
| BBa_K4161011 | csgB | http://parts.igem.org/Part:BBa_K4161011 |
| BBa_K4161012 | csgC | http://parts.igem.org/Part:BBa_K4161012 |
| BBa_K4161013 | csgE | http://parts.igem.org/Part:BBa_K4161013 |
| BBa_K4161014 | csgF | http://parts.igem.org/Part:BBa_K4161014 |
| BBa_K4161015 | csgG | http://parts.igem.org/Part:BBa_K4161015 |
| BBa_K4161016 | riboJ | http://parts.igem.org/Part:BBa_K4161016 |
| BBa_K4161017 | Neae: the intimin fragment for surface display | http://parts.igem.org/Part:BBa_K4161017 |
| BBa_K4161018 | pTlpA promotor C7 variant | http://parts.igem.org/Part:BBa_K4161018 |
| BBa_K4161019 | FE-36 linker | http://parts.igem.org/Part:BBa_K4161019 |
| BBa_K4161020 | JPS-G3 | http://parts.igem.org/Part:BBa_K4161020 |
| BBa_K4161021 | 3xFlag | http://parts.igem.org/Part:BBa_K4161021 |
| BBa_K4161022 | thr terminator | http://parts.igem.org/Part:BBa_K4161022 |
Group E composite segments
Group E's composite parts are designed to express surface protein-nanobody fusion proteins. A total of 4 composite parts are documented.
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161003 | csg-JMK-H2, Csg surface display system on E. coli Nissle 1917 | http://parts.igem.org/Part:BBa_K4161003 |
| BBa_K4161004 | intimin-20IpaD, Nanobody surface display system based on intimin | http://parts.igem.org/Part:BBa_K4161004 |
| BBa_K4161023 | csgA-JPS-G3-3xFLAG, Csg surface display system on E. coli Nissle 1917 | http://parts.igem.org/Part:BBa_K4161023 |
| BBa_K4161100 | Csg surface display system on E. coli Nissle 1917 | http://parts.igem.org/Part:BBa_K4161100 |
Group E vectors
Vectors pET30a and pUC19 are used for recombinant plasmid construction and protein expression.
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161024 | pET30a, empty vector | http://parts.igem.org/Part:BBa_K4161024 |
| BBa_K4161025 | pUC19, empty vector | http://parts.igem.org/Part:BBa_K4161025 |
L.lactis NZ9000 parts
Group L single elements
Single parts in Group L includes the following elements: Group L parts
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161600 | Usp45 nisin promoter | http://parts.igem.org/Part:BBa_K4161600 |
| BBa_K4161603 | AcmA3b | http://parts.igem.org/Part:BBa_K4161603 |
| BBa_K4161602 | 20ipaD | http://parts.igem.org/Part:BBa_K4161602 |
| BBa_K4161604 | signalling peptide in L.lactis | http://parts.igem.org/Part:BBa_K4161604 |
| BBa_K4161605 | Flag-tag | http://parts.igem.org/Part:BBa_K4161605 |
Composite element
The composite element of Group L includes Usp45-signaling peptide in L.lactis-Flagtag-20ipaD-AcmA3b.
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161600 | Usp45-signaling peptide in L.lactis-20ipaD-AcmA3b | http://parts.igem.org/Part:BBa_K4161997 |
Group L vector
Composite element
The plasmid backbone of Group L is pNZ8148, a plasmid suitable for L.lactis.
| Part code | Part name | Link |
|---|---|---|
| BBa_K4161601 | pNZ8148 | http://parts.igem.org/Part:BBa_K4161601 |