Attributions

Biology Team leader. In charge of wet lab organization, human practice organizations and wiki construction. Designed the overall topic and approach of the problem to tackle with synthetic biology design. Designer of E.coli csgA surface display and lab experiments of the E.coli group. In charge of outreach and secured funding for the project.

Yuxin Wang

Co-established the project topic. In charge of lab design and wet lab for the yeast group. Also assisted in Wiki writing and expert interviews.

Yunyi Ru

In charge of background research, E. coli group lab design, wet labs & wiki writing. Hosted journal clubs and science lectures for human practice.

Zhujun Yao

Designed Lactococcus lab procedures and is in charge of Lactococcus and yeast lab experiments. Conducted expert interviews for human practice.

Chang Shen

Proposed the project topic. Participated in plasmid design and workflow design of E.coli group. Participated in wet labs and background research. Helped conduct collaboration with XJLTU as a part of human practice.

Lingrui Lai

Responsible for wet lab design and experiment for yeast group. Contribute to Human Practice with Conference of China iGEMer Community.

Qizhou Jiang

Helped conduct human practices and wetlabs for the yeast group. Designed the safety and education Wiki pages and organized local high school educational activities.

Sihan Wang

Helped with background research. Designed lab detection protocols for the lactococcus group. Conducted wetlab experiments. Helped with human practice organization including journal club organization and wiki writing.

Yixuan Liu

Organized and chaired the iGEM2022 Journal Club Seminar ever since 2021. Actively participate in human practice part including designing and popularizing events in local collegiate schools. Help conduct wet labs.

Renwei Gao

The fund manager of the team, in charge of labware purchasing, helped with background research and survey distribution as a part of human practice.

Jingyun Jin

Helped design the survey as a part of human practice. Helped with E.coli wet lab experiments.

Modeling team

Zhenyu Xu

Modeling team leader. Organized protein structure and docking modeling. Organized mathematics modeling. Helped conduct human practice of expert interview, designed CCiC poster, edited the project presentation video. Shot and edited the promotion video. Wiki writing. In charge of outreach and secured funding for the project.

Sichang He (Steven)

The Wiki Engineer. Built the Wiki infrastructure with mdBook and authored all code for Wiki theming with designs and help from other team members, including all Handlebars for Wiki layout, all CSS (with Tailwindcss) for styling and animations, and all JavaScript for the table of contents and animations.

The technical support. Set up, owns, and maintains the GitLab repository for team communication and Wiki editing. Introduced Git and GitLab to the team and helped the team adapt to the workflow on GitLab. Taught the team how to use Visual Studio Code to write Markdown for the Wiki.

Protein modeling member. Helped with protein modeling using RoseTTAFold, Robetta, and PyMOL.

Jasmine Santos

Conceptualized cell surface display technology project during preliminary brainstorming and provided proof of concept through the review of related literature. Wrote the project abstract and refined the general methodology framework. Built protein models for candidate microbes via Alphafold and Haddock and evaluated fidelity of each model via quantitative metrics. Helped with mathematics modeling for the part of efficiency description. the fidelity of each model via quantitative metrics. Modeled the growth of chosen microbe model computationally.

Yuxiang He

Responsible for building a 3D model of the microcapsule. Created standard pages and provided technical support for the wiki. Provided technical support in using the Gitlab repository. Helped with protein modeling using PyMOL.

Advising support

Linfeng Huang

Overall project advising, principal investigator.

Jiani Chen

Oversee wet lab experiments and overall project advising.

Yang Yu

Oversee wet lab experiments. Helped optimize lab protocols and troubleshooting.

Funding support

Thanks to the generous funding support of Duke Kunshan University Natural Science Divisions, Wang-Cai Biochemistry Lab, Synear Food Molecular Biology Lab, DKU Innovation and Entrepreneurship Initiative, and Marcia B. France, DKU Dean of Undergraduate Studies. Thanks to free licenses provided by SnapGene.

Wet Lab support

Thanks to Dr. Jin Miao and Dr. Yingxue Li for advising our wet lab experiments. Thanks to our PI, Prof Linfeng Huang, for providing valuable advice and supervision throughout the project.

Communications support

Thanks to Prof. Floyd Beckford and Dr. Huansheng Cao of Duke Kunshan University, Dr. Neel Joshi from Northwestern University, and Dr. Jisheng Lin at Sinovac Biotech CO., LTD. for providing effective advice on our project design and development. Thanks to Prof. Dong Hyun Jo of Seoul National University College of Medicine for contributing to the education efforts of our project.

DKU iGEM Team Wiki