Team:CUG-China/Notebook - 2022.igem.org
Parts

Overview


This year we built a c-di-GMP biosensor based on the Ppel and FleQ system from P. aeruginosa. So we have to add these relative promotor and gene to the part registry. Among the 25 parts we designed this year, six of them are basic parts, the rest are composite parts. You can click on the part numbers to browse more detailed information. Most of the parts were used in our project, and there are a few part that we haven't finish the performance testing due to the time limit.

Parts List


Numbers Classification Description Length
BBa_K4242000 Coding fleQ 1473 bp
BBa_K4242001 Regulatory PcI 147 bp
BBa_K4242002 Regulatory PcI+Ppel 204 bp
BBa_K4242003 Coding fleN 843 bp
BBa_K4242004 Coding yedQ 1695 bp
BBa_K4242006 Composite fleQ+TT 1610 bp
BBa_K4242007 Composite RBS+fleN 861 bp
BBa_K4242008 Composite gfp+TT 857 bp
BBa_K4242009 Composite Pbad+RBS+fleQ+TT 2845 bp
BBa_K4242010 Composite PcI+RBS+fleQ+TT 1685 bp
BBa_K4242011 Composite Ppel+RBS+gfp+TT (pHYD-1) 1030 bp
BBa_K4242012 Composite PcI+Ppel+RBS+gfp+TT (pHYD-2) 1087 bp
BBa_K4242013 Composite PcI+RBS+fleQ+TT+ Pci+Ppel+RBS+gfp+TT (pHYD-3) 2780 bp
BBa_K4242014 Composite Pbad+RBS+fleQ+TT+ PcI+Ppel+RBS+gfp+TT (pHYD-B1) 3940 bp
BBa_K4242015 Composite Pbad+RBS+fleN+RBS+fleQ+TT+PcI+Ppel+RBS+gfp+TT (pHYD-B2) 4809 bp
BBa_K4242016 Composite Ptac+RBS+yedQ+TT (pSB3C5-A2) 3307 bp
BBa_K4242017 Composite Ptac+RBS+yhjH+TT (pSB3C5-A3) 2378 bp
BBa_K4242018 Composite RBS+fleQ+TT 1628 bp
BBa_K4242019 Composite RBS+fleQ+TT+PcI+Ppel+RBS+gfp+TT 3785 bp
BBa_K4242020 Composite RBS+gfp+TT 875 bp
BBa_K4242021 Composite RBS+yedQ+TT 1852 bp
BBa_K4242022 Composite RBS+yhjH+TT 923 bp
BBa_K4242023 Composite yedQ+TT 1832 bp
BBa_K4242024 Composite yhjH+TT 905 bp
BBa_K4242025 Regulatory Ptac+TT 1447 bp