Team:CUG-China/Notebook - 2022.igem.org
Notebook



  • Jan.
  • Feb.
  • Mar.
  • Apr.
  • May.
  • Jun.
  • Jul.
  • Aug.
  • Sep.
  • Oct.

Jan.

-Publicized IGEM competition and answered students' questions about iGEM.
-Recruited team members and interviewed.

Feb.

-Our team was officially established and held the first meeting!
-All student members received laboratory safety training.
-All student members learned the basic concepts and experiments of molecular biology to establish understanding of synthetic biology.

Mar.

-Brainstorm: Extensively read articles and listed potential topics.
-Visited Institute of Hydrobiology, Chinese Academy of Sciences to understand the application of synthetic biology.
-Determined the theme of our team!
-Assigned tasks to each team.

Apr.

-Art design group made team logo!
-All students learned from the previous excellent iGEM projects and reported at the weekly meeting.
-Designed biobricks.
-Reached a cooperation intention with XMU-China.

May.

-Art design group designed each member's cartoon image.
-Art design group and the dry experiment group learned how to make web together.
-Continued to learn the projects of previous excellent iGEM teams.
-Deeply insighted into notions of parts, devices, systems in synthetic biology.

Jun.

-Participated ARS2022 and shared our project at the conference.
-Submitted first version of safety form.
-Constructed the first plasmid pHYD-1 and inserted plasmid pHYD-1 into S.oneidensis MR-1 and E.coli BL21.

Jul.

-Held online communication with NYCU-formosa to learn their experience in modeling.
-Collaborated with Xiamen University.
-Attended Central China iGEM Communication.
-Constructed plasmid pHYD-2 and inserted plasmid pHYD-2 into chassis.
-Demonstrate the function of pHYD-2.

Aug.

-Be present at Conference of China iGEMer Community(CCiC) and introduced our project to other teams.
-Filmed and made our promotion video.
-Interviewed CATUG Biotechnology and visited Dr. Zunyang Ke.
-Constructed plasmid pHYD-3 and codon optimization of fleQ.
-Transformed plasmid pHYD-3 into BL21 and MR1.
-Tested the fluorescence using different biofilm dispersal agent.

Sep.

-Made our homepage!
-Inserted the pbad into fleQ and did pre-experiment.
-Utilized HPLC to detect the c-di-GMP level of pHYD-3 strain.
-Held a joint education conference with HUST-China.
-Partnership experiments.
-Transformed the plasmid in to BL21.
-Adapted the set of ordinary differential equations to better describe the translation and transcription of gene and solved the model by Runge-Kutta method.

Oct.

-Finish the construciton of team wiki.
-Documentaion of part improvement.