Parts Table

Category	Name	Biobrick #	Origin	Engineering Decisions	References	Phase
Promoters	Gmubi	BBa_K4201000	Glycine Max (Soybean)		1	Complete, Tested
Geranylgeranyl Pyrophosphate Synthases	Tc.cytoGGPPS	BBa_K4201001	Taxus canadensis	Codon optimized for G. max Location Tag Removal	2	Complete with P + T
	Gm.cytoGGPPS	BBa_K4201002	Glycine Max	Codon optimized for G. max Location Tag Removal	NIH Protein BLAST	Complete with P + T
	CrtE	BBa_K4201003	Pantoea anantis LMG 20103	Codon optimized for G. max	3	Complete with P + T
Taxadiene Synthases	CrtE-cyto-TDS-MBP	BBa_K4201004	Pantoea anantis LMG 20103 & Taxus brevifolia	Codon optimized for G. max Location Tag Removal Solubility Tag Added (MBP) GGPPS and TDs merged to promote substrate channeling	3	Complete with P + T
	cytoTDS2	BBa_K4201005	Taxus chinensis var. mairei	Codon optimized for G. max Location Tag Removal	4	Complete with P + T
	cytoTDS2-MBP	BBa_K4201006	Taxus chinensis var. mairei	Codon optimized for G. max Location Tag Removal Solubility Tag Added (MBP)	4	Under Construction
Hydroxylases	T5αOH	BBa_K4201007	Taxus baccata	Codon optimized for G. max Membrane associated sequence left intact	5	Complete with P + T
Acetyltransferases	TAT	BBa_K4201008	Taxus cuspidata	Codon optimized for G. max	6	Under Construction
Reporter Genes	GUS	BBa_K4201010	Multiple Sources	Codon optimized for G. max	7	Complete with P + T, Tested
	SF-GFP	BBa_K4201011	Aequorea victoria	Codon optimized for G. max	8	Complete with P + T, Tested
	RUBY	BBa_K4201012	Caryophyllal es Order	Codon optimized for G. max	9	Complete with P + T, Testing
Terminators	AtHSP	BBa_K4201009	Arabidopsis thaliana	Chosen for its small genomic size	10	Complete, Testing
Backbones	Amp BsaI	BBa_K4201013	NA	Blue/white gene occupying insert position Ampicillin/Carbenicillin Resistance BsaI and AarI/PaqCI Recognition Sites E. coli origin of replication	-	Complete for Use
	Chlor AarI	BBa_K4201014	NA	Blue/white gene occupying insert position Chloramphenicol Resistance BsaI and AarI/PaqCI Recognition Sites E. coli origin of replication	-	Complete for Use
	Kan BsaI	BBa_K4201015	NA	Blue/white gene occupying insert position Kanamycin Resistance BsaI Recognition Sites E. coli origin of replication	-	Complete for Use
Final Composite Parts (all coding sequences with Gmubi and AtHSP)	Crte-cytoTDS-MBP_RUBY	BBa_K4201016		See Basic Parts	-	In Testing
	Crte-cytoTDS2_RUBY	BBa_K4201017		See Basic Parts	-	Ready for Testing
	Crte-cytoTDS2-MBP_RUBY	BBa_K4201018		See Basic Parts	-	Under Construction
	CrtE_cytoTDS__T5αOH_RUBY	BBa_K4201019		See Basic Parts	-	Under Construction
	CrtE_cytoTDS2__T5αOH_RUBY	BBa_K4201020		See Basic Parts	-	Under Construction
	CrtE_cytoTDS2-MBP_T5αOH_RUBY	BBa_K4201021		See Basic Parts	-	Under Construction

References

De La Torre, C. M. & Finer, J. J. The intron and 5’ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues. Plant Cell Rep. 34, 111–120 (2015).
De La Peña, R. & Sattely, E. S. Rerouting plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).
Majer, E., Llorente, B., Rodríguez-Concepción, M. & Daròs, J.-A. Rewiring carotenoid biosynthesis in plants using a viral vector. Sci. Rep. 7, 41645 (2017).
Xiong, X. et al. The Taxus genome provides insights into paclitaxel biosynthesis. Nat. Plants 7, 1026–1036 (2021).
Edgar, S. et al. Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase. ACS Chem. Biol. 11, 460–469 (2016).
TAT - Taxadien-5-alpha-ol O-acetyltransferase - Taxus cuspidata (Japanese yew) | UniProtKB | UniProt. https://www.uniprot.org/uniprotkb/Q9M6F0/entry.
Jefferson, R. A. The GUS reporter gene system. Nature 342, 837–838 (1989).
Yang, S. et al. An efficient Agrobacterium-mediated soybean transformation method using green fluorescent protein as a selectable marker. Plant Signal. Behav. 14, 1612682 (2019).
He, Y., Zhang, T., Sun, H., Zhan, H. & Zhao, Y. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic. Res. 7, 1–6 (2020).
Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells. Plant Cell Physiol. 51, 328–32 (2010).
Lebendiker, M. & Danieli, T. Purification of Proteins Fused to Maltose-Binding Protein. Methods Mol. Biol. Clifton NJ 1485, 257–273 (2017).