There have been several contributions made by CSMU_Taiwan this year. First, we added information to some existing Parts according to what we learned from the literature. Besides, we also created some functional models regarding AID expression and antibody collection. What’s more, we designed a suitable bioreactor, which can help with antibody collection and cell culture. Last but not least, we dedicated ourselves to promoting our project and synthetic biology in novel ways! It is truly our pleasure to share these things with people worldwide, and we firmly believe that all the above contributions can somehow help future iGEM teams!
Modeling always plays an essential role in experiments, and so as our project. We have developed two models to assist Wet Lab by utilizing the MATLAB application. On the one hand, we built the culture and production model to optimize hybridoma cultivation best. On the other hand, we constructed the AID-expression model that demonstrates the Tet-On and dNES mechanism in our modified sequence. Both models help us predict and optimize the experiments. Their functionality truly allows future iGEM teams to take advantage of it if they are interested in similar things. For more information, please see the Model page.
To replace the staffing-consuming fed-batch culture method, we designed a bioreactor for CHO cell perfusion culturing with cell retention microfluidic device inside. We also integrate some unique designs from other creatures. These bio-inspired designs may boost the bioreactor's efficiency and the public's attention to endangered creatures like giant oceanic manta rays (Mobula birostris). Last but not least, we practice a fast quality examination of produced antibodies from the bioreactor by designing a self-assembled monolayer biosensor. Our bioreactor has full functionality, ultimately attaining cell culturing, efficiency promoting, and quality monitoring in one design. For more information, please see the Hardware page.
Our educational activities started from arousing kids’ interest in synthetic biology to giving students and younger adults an insight into our project. We spared no effort to extend to the scope of the audience so that every individual can have a chance to understand synthetic biology as well as iGEM, and we took many novel ways to reach our goal. For more information, please see the Education page.
