Overview

Early on in our project, from our human practices discussions, we found out the pBAD promoter from the araBAD operon inducible by arabinose. The wild type promoter is extremely long, so we decided to research possible optimisation strategies, more details can be found on the part improvement page.

We decided to take a combinatorial approach to the part improvement, testing out different combinations of improvement strategies we found. This led us to producing a library of new pBADs - our part collection. These all have the significant advantage of being much shorter, and have variable strengths and leakiness. Full details are available on the linked registry pages. Note that, as the Registry does not generally allow an entry with multiple sequences, we gave the sequence of pBAD_AP2 only as a representative (shows significant improvement), but all remaining sequences are also given below for completeness.