human-practices
Official account number
We created WeChat official account and bilibili video account, and we introduced iGEM and our team. We also shared our project design and wrote articles to introduce the knowledge of synthetic biology to the public.
School propaganda
We communicated with other teams at school and explained their respective projects. The focus of our communication is to introduce the experimental purpose, experimental process, experimental scheme, experimental conclusion and future prospect of our project. Let our classmates and teachers around us know our project better.
Feedback:
Since the electrode of our biosensor for copper ion detection was carbon rod in the first generation design, the teacher asked whether we could increase the adhesion of engineering bacteria to the electrode to increase the voltage to make the result more obvious, so we replaced our carbon rod with carbon felt after discussion with the teacher. The modified electrode makes it easier for engineering bacteria to attach to the electrode, and the voltage produced by the biosensor is more obvious and easy to distinguish, which improves the performance of our biosensor
Enterprise access
We also went to BGI to explain our project. BGI has rich experience in genetic modification of bacteria.
Feedback:
After listening to the introduction of our project, the researchers there suggested that Escherichia coli has a compact and low permeability membrane, which limits the transfer of electrons from cells to electrodes. We can add porin-related genes to enhance the permeability of Escherichia coli cell membrane.
We have consulted a large number of literatures and found that oprF porin gene can increase the permeability of Escherichia coli cell membrane. Therefore, we synthesized porin gene (oprF) by direct DNA synthesis, and regulated it by constitutive promoter to ensure the continuous expression of this gene, thus constructing our amplification system. The expression of porin gene greatly improves the performance of our biosensor