Attributions
External Attributions

Jing Zhang, Tongji University of China
Our PI who helps us evaluate the feasibility of the project and make recommendations.

Dr. Ye Leng, Tongji University of China
Provide guidance and help manage some team affairs related school works.

Dr. Yaozu Xiang, Tongji University of China
Give advice on literature research, bromelain expression and directed evolution.

Dr. Changsheng Du, Tongji University of China
Provide advice on expression plasmid construction. Guide us some skills in experiment and provide some experimental resources.

Dr. Zhanyun Guo, Tongji University of China
Give advice on our project and help us revise the idea of the narrative project.

Dr. Chunguang Wang, Tongji University of China
Identify problems with the project to help us make some adjustments and guide us to pay attention to the focus of our project.

Dr. Mengjie Zhang, Tongji University of China
Help us to deal with some problem encountered in chassis selection, modification and bromelain experession.

Dr. Jiahao Shi, Tongji University of China
Provide advice on gene editing in B.subtilis.

Dr. Ping Li, Tongji University of China
Guide us some plasmid transformation and B.subtilis culture skills.

Dr. Chong Li, Tongji University of China
Make recommendations in the stage of brainstorming and topic selection.

Dr. Shaorong Gao, Tongji University of China
Provide laboratory resources and materials for us.

Libo Xing, Tongji University of China
Help to manage team affairs mainly in reimbursement.

Yunzhe Kang, Tongji University of China
Our adviser who helps us choose project topic and evaluates the feasibility of the project.

Siyuan Li, Tongji University of China
Give advice on our project and mainly help us handle with difficulties met in directed evolution.

Yanfeng Liu, China Pharmaceutical University
Give advice on Molecular Modeling and experiment.

Jixuan Lin, Tongji University of China
Give suggestions on project topic selection and track selection, and share experience with us.

Yiying Zhang, Tongji University of China
Give suggestions on project topic selection.

SUNSON limited company
Tells us the current production problems of bromelain and discusses some safety issues we should pay attention to.

DOING-HIGHER biotechnology limited company
Tell us about the market for bromelain preparations and evaluate our program.

PANGBO Enzyme limited company
Tell us the production status of bromelain enzyme preparation and promote the development of our project.

Hunan TAIREN Pharmaceutical limited company
Tell us the market states of bromelain in the field of medicine, and promote the topic selection of our project.

Team Attributions

Jingxian Cai
The leader of our team. She was a member of experiment group and was mainly responsible for chassis selection. Besides, she also coped with many matters of our team and details in project such as reminding deadlines, presiding over regular meeting, communicating with other teams, writing safety form, combing the wiki outline, preparing for presentation and so on. She did a good job in organizing team members to work in an orderly manner.

Jiaying Wen
When the team was just established, several other students and she came up with the project idea about stem bromelain. She was mainly responsible for the Dry Lab in the subsequent project implementation. These jobs in detail include the directed evolution of stem bromelain, the docking calculation of stem bromelain and its variant with BAEE, the molecular simulation, RMSD value calculation and so on.

Zhenxiang Guo
Leader of the late wet lab team. She was primarily responsible for the experimental design of the microbial chassis. She undertook most of the laboratory maintenance during the experiments, including but not limited to reagent purchases, liaising with professors and mentors.

Jinyi Ye
In the team, she was mainly responsible for wet lab. In detail, these jobs included the design and selection of parts and expression vectors in both Escherichia coli and Bacillus subtilis through detailed literature reading during the quarantine. As for the experiment part, she also participated in the construction of vectors, expression of the recombinant protein, characterization of the mutated variants and so on.

Keyao Zhao
A member of Tongji_China. Mainly choose suitable parts and design experiment. Meanwhile, ask some questions to promote partnership with other teams. Take on part of the Hp work.

Huiwen Cheng
Coming from School of Chemical Science and Engineering. He was a member of experiment group and was mainly in charge of protein purification and physicochemical properties determination. He helped to draw up extensive experiment protocols which were indispensable in the whole project.

Kingshuen Lu
Mainly responsible for education and public engagement: organized courses and meeting, designed card game and questionnaires. Participated in the topic selection and help the team determine technical roadmap and application scene. Participated in the implementation part, designed fermentation tank and the preservation plan of the enzyme preparation. Participated in the lab work and was responsible for optimizing the induction conditions.

Fang Yi
She was mainly responsible for the Implementation part of the project. At the same time, she was also responsible for writing wiki and helped other sections do some research work in the early stage of the project.

Guosheng Tong
He was responsible for Integrated Human Practice. He was a member of the experimental group responsible for the design of proof-of-concept experiments and the design of characterization experiments. He was also responsible for team registration payment and Jamboree payment.

Peizhong Gao
He is a designer. He was a member of Design&Wiki group and was charge of visual design and PV. Besides, he was ready to offer some creative ideas for the project. All in all, he did a great job in design and innovation.

Yanbin Yang
The designer of our team. He was mainly responsible for the design part of the project, determining the design style and designing the main visual elements with another designer. In addition, he also continued to work closely with other team members and undertakes animation, web design and illustration tasks.

Special Thanks

All members of team Tongji_China 2021,

Tongji University,

School of Life Sciences and Technology of Tongji University,

College of Chemical Science and Engineering of Tongji University,

Gao Lab,

SJTU-BioX-Shanghai,

CUHKSZ,

ZJU-China,

FAFU-CHINA.