Three-dimensional visualization softwares were a key component needed to create this project successfully. Programs such as PyMOL were highly important pertaining to the wet lab’s work seeing as they needed to model DNA strands, proteins, binding proteins and more. The 3D models such as the Bst DNA polymerase and similar proteins helped compare similarities between different proteins to then determine what challenges we needed to overcome to design a modified polymerase.

On top of the 3D modelling done for the wet lab portion of the project, the dry lab team worked on hours of modelling to fully produce the final functioning kettle device. With the main program being Solidworks, every single 3D printed or manufactured part was designed, tested, and toleranced in the program. For the device to work, every part needed to be built from scratch, assembled to make sure the parts fit together, and then toleranced properly for successful machining. Not only did this take a considerable amount of time, but it also took many different iterations, 3D printed tests, and lots of fails along the way. The parts shown below contain every single part used to build the physical kettle device as well as the housing for its filter.