Wet Lab Team
The team was led by Yueqiu Bai , and consisted of members Yu Wang, Yanqiu Tian, Jiayi Cheng, Yiwei Li, Xiangcai Liu,Haonan Shao.
The overall technical route of the experiment was constructed by Yu Wang and Yueqiu Bai in the early experiment, and Yu Wang and Haonan Shao completed the molecular biology experiment.
The induction of protein expression was carried out by Yanqiu Tian. Jiayi Cheng was responsible for the sample preparation and handed over to Xiangcai Liu for SDS-PAGE verification. The HPLC detection was carried out by Yueqiu Bai. Yiwei Li completed the work related to the suicide module.
Human Practices Team
The team was headed by Jiayi Cheng, and consisted of members Fankang Kong, Fangyan Li ,Chenyang Li and Siqing Wang.
Jiayi Cheng is responsible for the preliminary writing and sorting of the manuscript, contacting and organizing events, revising and approving manuscripts. Fankang Kong,Fangyan Li and Chenyang Li embellished and typeset the manuscript. Siqing Wang records our activities by taking photos. In addition, Fankang Kong and Fangyan Li are responsible for the translation of the manuscript, and Fangyan Li and Chenyang Li are responsible for the writing and art of the tweets.
Wiki Team
The team was headed by Yanqiu Tian, and consisted of members Sikai Mi and Xvhao Feng.
Sikai Mi and Xvhao Feng were the wiki builders and content rendering advisors, while Yanqiu Tian was responsible for docking other teams.
Modelling Team
The team was headed by Xiangcai Liu, and consisted of members Jiefu Zheng and Shiqi Wei.
Their work consists of two parts. First part is molecular docking work, predicting the catalytic activity site of the target protein and providing a basis for subsequent protein transformation. Second part is modeling and predict of the diffusion phenomenon of strains into natural water bodies.
Art Design Team
The team was headed by Yueqiu Bai, and consisted of members Junbo Liu and Xiaohan Tang.
Junbo Liu and Xiaohan Tang led the visualization process in promotion video and Wiki production ensuring our project are looked as appealing online as it did in person. Yueqiu Bai was promotion video director.
Advisors
Jinlu Hu (Primary Principal Investigator)
Jinlu Hu (Primary Principal Investigator)
General project support
Wet lab design
Presentation coaching
Weining Niu (Secondary Principal Investigator)
Wet lab design
Difficult technique support
Jun Wang (Instructor)
Difficult technique support
Science experiment design
Jun Wang, Assistant Research Fellow, Experimental Teaching Center, School of Life, Northwestern Polytechnical University.
Dr. Wang provided guidance for protein heterologous expression in E. coli and enzyme activity assay. He also provided advice for creation of suicide plasmids, measurement of PAH degradation and some microbiological experiments.
Shanfeng Jiang (Instructor)
Difficult technique support
Shanfeng Jiang, Assistant Research Fellow, Experimental Teaching Center, School of Life Sciences, Northwestern Polytechnical University.
Dr. Jiang provided adequate safety instructions in preparation for chemical reagents and operating for experimental device. He also provided help in laboratory biosafety.
Yuan Gao (Instructor)
Assistant Research Fellow, Experimental Teaching Center, School of Life Sciences, Northwestern Polytechnical University.
Dr. Gao provided common experimental technique support and experimental reagent and material management for this project.
Jie Shang(Instructor)
Assistant Professor, School of Life Sciences, Northwestern Polytechnical University.
Dr. Shang provided guidance for analytical method on HPLC and LCMS. He also shared his knowledge about the chemical property of organic pollutant.
External Attributions
Dr. Qiang Wang
Dr. Qiang Wang
Professor, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University
Dr. Wang gave us feedback on our overall project design and module design for exogenous expression in E. coli. He also provided important advice for future releases of our strains into the environment.
Dr. Ming Li
Professor, College of Natural Resources and Environment, Northwest Agriculture and Forestry University.
Dr. Li shared knowledge about the current status of organic pollutants in the environment and their impact on the ecology. He provided the idea of co-expressing the ligninolytic enzymes to degrade PAHs in the environment. He also gave advice on what to look out for and how to deal with possible obstacles.
Dr. Xin Zhang
PhD in Genetics, Assistant Professor, College of Life Science, South-Central Minzu University.
Dr. Zhang provided guidance for molecular docking between the ligninolytic enzymes and phenanthrene. She also shared her research data for our modeling team to make molecular docking models of the ligninolytic enzymes.
Dr. Aipeng Li
Associate Professor, School of Chemical Engineering and Technology, Xi’an Jiaotong University
Dr. Li provided guidance for our wet lab team by meeting with us to discuss assay design. He also shared his knowledge about the kinetic determination of enzyme activity.