Internal Attribution
The following is a list of our members, who worked their utmost effort for the project.
Members
Yi Kai CHAN, Deja
- Researched on mechanism and optimization of cell-free system.
- Designed and conducted experiments for optimization, functionality tests of cell-free system, cycle 0 and cycle 1 parts.
- Researched on hardware components
- Designed and animated promotion video.
Man Yee NGAI, Coby
- Researched on mechanism and optimization of cell-free system.
- Designed and conducted experiments for optimization and functionality tests of cell-free system.
- Designed logo, and designed and animated promotion video.
Qingyan LI, Grace
- Researched on mechanism and optimization of cell-free system.
- Designed and conducted experiments for optimization and functionality tests of cell-free system.
Mridini Prashant MEHTA
- Researched on mechanism and optimization of cell-free system.
- Designed and conducted experiments for optimization and functionality tests of cell-free system.
- Researched on hardware components and protocol writing.
Janviere YAU
- Designed cycle 1 circuit.
- Cloned pre-designed circuit components in cycle 1 into competent E. coli strains.
- Designed and conducted experiment for the characterization of degradation rescue and induced degradation system.
- Conducted experiments for interlab.
Leon Ritchie SALIM
- Cloned pre-designed circuit components in cycle 0 into competent E. coli strains
- Design and conduct experiment for the characterization of rDAO, oxyR, oxySp and katGp parts.
- Code for Sobol Analysis (Global Sensitivity Analysis)
- Developed the differential equations for cycle 0.
Sherman CHAU
- Conducted research and design of components for cycle 0 circuit
- Cloned pre-designed circuit components in cycle 0 and cycle 1 into competent E. coli strains
- Troubleshooted and optimized protocols for general biochemical methods regarding DNA amplification, DNA extraction, and bacterial transformation
Man Ping LEE, Angie
- Researched on possible kill-switch mechanisms
- Designed and conducted experiments on the detection of protein expression of oxyR, rDAO and TEV protease in E. Coli and cell-free system
- Troubleshooting cell lysis protocol
Jihye PARK, Lily
- Researched on potential biosensor designs.
- Designed overall hardware experiments.
- Conducted compatibility experiment, ninhydrin, and swab recovery rate experiment.
Dinara SYAHIDA
- Secretary of the HKUST iGEM 2022 team.
- Researched on potential biosensor designs.
- Assisted in planning and conducting compatibility experiment, ninhydrin optimisation and swab recovery rate experiment.
Payal GURUNG
- Researched on potential biosensor designs.
- Designed hardware experiments.
- Conducted compatibility experiment, ninhydrin optimisation, and swab recovery rate experiment.
Sneha Pritam RAJESHIRKE
- Researched on potential biosensor designs.
- Designed hardware experiments.
- Conducted compatibility experiment, ninhydrin optimisation, and swab recovery rate experiment.
Jiyeon SON
- Researched on potential biosensor designs.
- Assisted in planning and conducting compatibility experiment, ninhydrin optimisation, and swab recovery rate experiment.
- Designed and organized UI/UX of the Wiki and assisted to code actual wiki.
Daye KWON, Audrey
- Developed Cycle 1 differential equations and analyze modeling outputs.
- Assisted in the experiments and analysis of the CFS optimization model.
- Designed social media posts and goods for public engagement.
Dowon KWAK, Jacob
- Coded for the Morris and eFAST Method for global sensitivity analysis.
- Designed the CFS optimization model and collect the data through experiments.
- Assisted in the mathematical modeling of cycles.
Jintaek OH
- Developed the CFS optimization model.
- Assisted mathematical modeling for cycles.
- Organized and coded most of the actual wiki as Head Developer
Kwan To CHAN, Toby
- Initiated the 2D and 3D bacterial growth modeling to support the biosensor prototype.
- Assisted in the development of the eFAST method.
Leong CHENG
- Communicated with various industry stakeholders and experts.
- Researched on local & global seafood market, bioamines measurement and regulations.
- Developed the backstory.
- Adjusted modeling outputs for Cycle 0 and Cycle 1.
Minjin GU
- Contacted other iGEM teams to stay connected with other teams and promote our project.
- Designed the posters, pamphlets, booklets used to promote our project as well as team merchandise.
- Designed and organized UI/UX of the Wiki and assisted to code actual wiki.
Hou Sang YAM, Ivor
- Connected with different high schools and initiate educational workshops.
- Collaborated with overseas iGEM teams for promoting iGEM and synthetic biology.
- Create recap videos about the educational workshops and the symposium.
Advisors
Ou Ning HSIU, Owen
Circuit and laboratory experiment consultant
Qian Xin LI, Andreas
Hardware and circuit experiment consultant
Siu Fung, NG
Consulted and assisted troubleshooting of developed models
Diya, AGRAWAL
Troubleshooting
Chloe NG-BROSSARD
As the IHP consultant, provided guidance in reaching out to industry experts and other iGEM teams as well as managing logistics.
External Attribution
The following is a list of professors, and people from different organizations who helped to improve our project and shapen our project direction.
Mr. Mark Van Asten
He provided suggestions for further hardware improvement and possible integrations with existing quality control measures.
Morgan Cousin (M&C)
They confirmed the necessity of a quick testing kit for seafood spoilage in the market and provided advice regarding our target audience and hardware design.
Mr. Chris Sannito (University of Alaska Fairbanks)
He provided insights regarding the actual implementation of our product in the HACCP framework
Mr. Henry Yip
He provided us with a comprehensive understanding of the current regulations for fish quality inspection in Hong Kong and its existing gaps.
Mr. Willem Landman
He described how quality control is implemented in Food & Beverages companies in Hong Kong and explained how our product might be useful in the Hong Kong market.
Dr. Peter Luk
He explained common ways of sampling fish for quality control and provided suggestions for our hardware design.
Dr. Quentin Fong
He helped us refine the target audience by sharing his years of experience in the fish quality control sector.
Professor Michael Lam
He provided advice for the overall project as an entrepreneur who has started a similar project.
Ms. Abigail Chu
She provided suggestions to refine our initial project direction and gave us an overview of how the fish retailers in Hong Kong maintain the freshness of their fish.
Mr. Taishi Tonooka
He provided suggestions in optimizing cell-free system and advice on the appropriate rehydration method of cell-free system.
Dr. Henry Lam
He provided guidance in modeling and parameter configurations
LAI Yong (The Hong Kong University of Science and Technology)
He offered useful advice on the use of degradation tags and emphasized the need for an amplification system in our genetic circuit.
HUNG Siu Chun (The Hong Kong University of Science and Technology)
He offered useful information on the current methods of bioamine and H2O2 detection. and emphasized the importance of a positive control in a test kit.
ZHENG, Bo (Shenzhen Bay Laboratory)
He emphazed the importance of incubation temperature of our cell-free reaction, which inspired us to investigate the respective effects of different temperatures. Provided us valuable suggestions on our hardware design.
SUN, Fei (The Hong Kong University of Science and Technology)
He attached other applicational possibilities to our project idea. Broadened our horizon with state-of-art hardware designs.
Michael Jewett (Northwestern University)
He offered essentially detailed and useful advice on the procedures of crude cell extract preparation, which improved the effectiveness of our homemade cell-free system by a considerable level.
Mr. Martin Dijk (Seafood Friday)
He explained the industry practices related to quality inspection standards (and the lack of it) and how Seafood Friday deals with quality compromises.
Hong Kong Science Museum
They collaborated with us on hosting a face-to-face public session on Synthetic Biology as part of their Biodiversity workshop.
Other iGEM Teams
Thank you for sharing their own projects and providing their suggestions to ours during Zoom and face-to-face sessions.
- University of Patras
- National Cheng Kung University
- IISER Tirupati
- Waseda University
- University of Rochester
- Manipal Institute of Technology
- The Chinese University of Hong Kong
- National Yang Ming Chiao Tung University
- National Yang Ming Chiao Tung University_Formosa
- University of Toronto
- The University of Hong Kong
- City University of Hong Kong
- Hong Kong Carmel Park U Secondary School
