Part Collection
All parts and constructs we used in our project can be found on this page. Here you can find parts that we designed:
Improved basic parts of the cellulose operon from Komagataeibacter xylinus
- BBa_K4369003 [endo-beta-1,4-glucanase (CmcAx) and the endoglucanase (ccpAX)]
- BBa_K4369004 [acsAB- catalytic and regulatic subunit of the cellulose synthase]
- BBa_K4369005 [acsCD- subunits of the cellulose operon]
- BBa_K4369006 [bglxA- ß-galactosidase, ancillary protein in cellulose operon]
Composite part of the complete cellulose operon
- BBa_K4369001 [Complete K. xylinus cellulose operon (adjusted for E. coli)]
Alg8 gene for overexpression
- BBa_K4369000 [glycosyltransferase in alginate operon]
We also made great use of the parts of the distribution kits of the last year, not only the parts that we designed. This were the parts we used for our transformations and PCRs:
Vector (pSB1C3) and arabinose promoter
Catalytic subunits from the cellulose operon of K.xylinus
- BBa_K1321334 [acsAB- catalytic and regulatic subunit of the cellulose synthase]
- BBa_K1321335 [acsCD- subunits of the cellulose operon]