Basic Parts

Group	Name	Type	Function
1	BBa_K4117002	CYP2C9	The gene CYP2C9 encodes the enzyme CYP2C9, which not only take part in the reaction where the Δ9-THC hydroxylates into 11-OH-Δ9-THC, but also catalyzes the oxygenization reaction from 11-OH-Δ9-THC to THC-COOH
	BBa_K4117022	CYP2C19	The gene CYP2C19 encodes the enzyme CYP2C19, which takes part in the reaction where the Δ9-THC hydroxylates into 11-OH-Δ9-THC.
	BBa_K4117222	UGT1A3	The gene UGT1A3 encodes the enzyme CYP2C19, which catalyzes the glucuronidation of the former substrate, increases the metabolite's water solubility, thereby
2	BBa_P1010	ccdB	ccdB can express an endotoxin that promotes bacterial suicide.
	BBa_K3402000	yeGFP	The yeGFP can express green fluorescence protein, which can be used as a report gene to characterize the strength of promoters.
	BBa_K4117666	RBS	This part act as an RNA thermometer which can be used for temperature-sensitive post-transcriptional regulation. Translation is initiated at 37°C and suppressed below 37°C.
3	BBa_K4117777	C-phycocyanin beta subunit	This gene encodes the beta subunit of Nostoc flagelliforme NX-09 C-phycocyanin. C-phycocyanin is a blue pigment protein that commonly used as natural dye in food.
	BBa_K4117778	C-phycocyanin alpha subunit	This gene encodes the alpha subunit of Nostoc flagelliforme NX-09 C-phycocyanin. C-phycocyanin is a blue pigment protein that commonly used as natural dye in food.
	BBa_K4117779	anti-THC pmrB	We use an antibody sequence that binds to THC to replace the extracellular binding domain of the receptor pmrB in the PmrCAB system, enabling it to open a downstream pathway by binding to THC. So we can use this part to make the bacteria respond to THC.

Composite Parts

Group	Name	Type	Function
1	BBa_K4117008	detect THC and secrete CYP2C9	This part detects THC and secretes CYP2C9 outside the cell.We combined pmrA(BBa_K3611003),anti-THC pmrB(BBa_K4117779) ,rrnB T1 Terminator(BBa_K3257020),pmrC promoter(BBa_K1459006) ,OmpT(BBa_K3805532) ,CYP2C9（BBa_K4117002），T7 terminator(BBa_B0012) .When the bacteria detects THC, CYP2C9 is formed .
	BBa_K4117088	detect THC and secrete CYP2C19	This part detects THC and secretes CYP2C19 outside the cell.We combined pmrA(BBa_K3611003),anti-THC pmrB(BBa_K4117779) ,rrnB T1 Terminator(BBa_K3257020),pmrC promoter(BBa_K1459006) ,OmpT(BBa_K3805532) ,CYP2C19（BBa_K4117022），T7 terminator(BBa_B0012) .When the bacteria detects THC, CYP2C19 is formed .
	BBa_K4117888	detect THC and secrete UGT1A3	This part detects THC and secretes UGT1A3 outside the cell.We combined pmrA(BBa_K3611003),anti-THC pmrB(BBa_K4117779) ,rrnB T1 Terminator(BBa_K3257020),pmrC promoter(BBa_K1459006) ,OmpT(BBa_K3805532) ,UGT1A3（BBa_K4117222），T7 terminator(BBa_B0012) .When the bacteria detects THC, UGT1A3 is formed .
2	BBa_I0500	Inducible pBAD/araC promoter	pBAD is an E.coli promoter that is tightly controlled by inducer L-arabinose and repressor Arac.
	BBa_K302032	mazE&mazF	mazE&mazF encodes the liable antitoxin MazE and toxin MazF in Bacillus Subtilis, which are used to provide a toxin-antitoxin kill switch.
3	BBa_K4117780	detect THC and secrete C-phycocyanin	This part detects THC and secretes C-phycocyanin outside the cell.We combined pmrA(BBa_K3611003),anti-THC pmrB(BBa_K4117779) ,rrnB T1 Terminator(BBa_K3257020),pmrC promoter(BBa_K1459006) ,OmpT(BBa_K3805532) ,C-phycocyanin alpha subunit(BBa_K4117778) ,GS linker,His-tag,OmpA(BBa_K103006),C-phycocyanin beta subunit(BBa_K4117777) ,S-tag and T7 terminator(BBa_B0012) .When the bacteria detects THC, C-phycocyanin is formed and C-phycocyanin can be observed as blue by the naked eye.