Wet Lab

Part Collections

For the purposes of our project, we synthesized Ribosome Binding Sites (RBS) in order to simulate different weights for our perceptron algorithm. We built them with the guidance of the RBS calculator, and we characterized them through our experiments.

You can find more about it in our Part Collection tab.

New Basic Parts

We generate regulatory sequences, RBS sequences, coding sequences and spacer sequences for the purposes of our project, and we believe that they are a valuable contribution to the iGEM community.

You can find more about it in our Parts tab.

New Composite Parts

Our New Composite Parts include senders and receivers circuits in order to help future iGEM teams to simulate a perceptron algorithm in their projects.

You can find more about it in our Parts tab.

Improvement of a Part

The improved part is: BBa_K4294559 The improvement is upon the part: BBa_B0034

You can find more about it in our Improve tab.

mNeonGreen

Enriching the iGEM Registry of Standard biological parts with mNeonGreen information (BBa_K4294410 - mNeonGreen Codon Optimised for E Coli & BBa_K1761003 - mNeonGreen)

Fluorescent proteins are particularly useful genetically encoded tags to visualise gene products and cellular compartments in living cells and organisms. In order to improve emitted signals and make them versatile tools, diverse fluorescent protein variants with different spectral and photophysical properties have been developed. The most famous among them is the Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, who revolutionised cell and developmental biology research. However, in 2013 Shaner and collaborators engineered the amphioxus Branchiostoma lanceolatum multimeric yellow fluorescence protein (LanYFP) to produce the monomeric mNeonGreen protein.

mNeonGreen consists of 237 amino acids, which translates into 26.6 kDa molecular weight. She presents optimum absorbance at 506λ(nm) and optimum emission at 517λ(nm).



mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein at the time. Actually, it is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs.

mNeonGreen excitation and emission peaks are slightly shifted toward higher wavelengths as compared to classical GFPs, but remain compatible with standard “GFP” filter sets used for microscopy. Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged either with standard green fluorescent protein filters or yellow fluorescent protein filters, with no or minimal reduction in emission readout, respectively.

mNeonGreen is evolutionarily distant from jellyfish-derived fluorescent proteins. At sequence level, mNeonGreen shares just 20-25% sequence identity with common GFP derivatives.



It seems to be more stable and less sensitive to laser induced bleaching than EGFP. Therefore, mNeonGreen is particularly suitable for confocal and super resolution microscopy, especially when fusion proteins are investigated, which are expressed at low levels. It has already been expressed in multicellular organisms like C.elegans.